92-kDa type IV collagenase and TIMP-3, but not 72-kDa type IV collagenase or TIMP-1 or TIMP-2, are highly expressed during mouse embryo implantation
| Autor: | I Thesleff, Bjorn R. Olsen, C Sahlberg, S S Apte, I Leivo, Paula Reponen, Karl Tryggvason |
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| Rok vydání: | 1995 |
| Předmět: |
medicine.medical_specialty
Stromal cell In situ hybridization Biology Basement Membrane Mice Internal medicine Decidua medicine Animals Collagenases Embryo Implantation Glycoproteins Tissue Inhibitor of Metalloproteinase-3 Tissue Inhibitor of Metalloproteinase-2 Metalloproteinase Metalloendopeptidases Proteins Trophoblast Tissue Inhibitor of Metalloproteinases Embryo Embryonic Tissue Molecular biology Decidual reaction Extracellular Matrix Trophoblasts Mice Inbred C57BL Molecular Weight Endocrinology medicine.anatomical_structure Enzyme Induction Protein Biosynthesis embryonic structures Mice Inbred CBA Collagenase Female Developmental Biology medicine.drug |
| Zdroj: | Developmental Dynamics. 202:388-396 |
| ISSN: | 1058-8388 |
| Popis: | Expression of 72 kDa and 92 kDa type IV collagenases and the metalloproteinase inhibitors TIMPs 1, 2, and 3 was studied by in situ hybridization in implanting mouse embryos of days 5.5 to 7.5. The 92 kDa type IV collagenase was strongly expressed in invading trophoblasts, signals above background not being observed in the embryonic proper or placental tissue. In contrast, signals above background were not seen for the 72 kDa enzyme in any cells of the implantation region, including trophoblasts and stromal cells of the decidual tissue. Only cells in the mucosal stroma outside the decidual region displayed some expression. TIMP-3 was intensily expressed in maternal cells in the area surrounding the invading embryonic tissue. No expression was observed for TIMP-1 or TIMP-2 in the embryo proper, trophoblasts, or the area of the uterine decidual reaction. Weak signals appeared for TIMP-1 only in the circular layer of myometrial smooth muscle and in some uterine stroma cells distant from the site of embryo implantation. The results suggest a central role for 92 kDa type IV collagenase and TIMP-3 in the extracellular proteolysis associated with implantation of the early embryo. |
| Databáze: | OpenAIRE |
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