Hematein chelates of unusual metal ions for tinctorial histochemistry
| Autor: | AA Smith |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Chromium
Histology Metal ions in aqueous solution Inorganic chemistry Ionic bonding Metal chemistry.chemical_compound Polymer chemistry Animals Humans Chelation Hematein Hematoxylin Chelating Agents Cell Nucleus Ions Staining and Labeling Mordant General Medicine Chrome alum Mitochondria Staining Medical Laboratory Technology chemistry Metals visual_art visual_art.visual_art_medium Alum Compounds Bismuth |
| Zdroj: | Biotechnic & Histochemistry. 85:43-54 |
| ISSN: | 1473-7760 1052-0295 |
| Popis: | Hematoxylin is oxidized easily to hematein, an excellent stain for metal ions. If it already is bound to a substrate, the metal ion becomes a mordant linking the dye to the substrate. Metal ions added to hematein in solution are chelated by the hematein to form a lake. Most of these chelates stain animal tissues. They usually are bound to the tissue by a combination of hydrogen bonding of the hematein and ionic bonding of the metal ion. When binding of the lake to the tissue occurs by way of the metal ion, the metal ion is a mordant. Mordant staining often is specific. Chromium hematoxylin binds to strong acids; it can be made selective for protein-bound sulfonic acids. Zirconyl hematoxylin is selective for acidic mucins. Mucihematein can be made selective for all acidic mucins or for sulfomucins alone. Bismuth hematoxylin appears to be selective for the guanido group of arginine and there is some evidence that the bonding is covalent. Although it is not a histochemical stain, copper-chrome hematoxylin is an excellent stain for organelles with double membranes, i.e., mitochondria and nuclei. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |