Potential role for CD63 in CCR5-mediated human immunodeficiency virus type 1 infection of macrophages
| Autor: | Ronald G. Collman, William A. O'Brien, Jana J. von Lindern, Georges Herbein, Julie M. Decker, Daniel R. Rojo, Christine Yeramian, Anjali Singh, Todd C. Pappas, Monique R. Ferguson, Cheng Deng, Kathie Grovit-Ferbas |
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| Rok vydání: | 2003 |
| Předmět: |
Receptors
CCR5 medicine.drug_class Immunology HIV Infections Platelet Membrane Glycoproteins Monoclonal antibody Microbiology Polymerase Chain Reaction Cell Fusion Antigen Antigens CD Virology medicine Macrophage Humans Lymphocytes Cells Cultured Cell fusion biology Tetraspanin 30 Macrophages Antibodies Monoclonal Virus-Cell Interactions Real-time polymerase chain reaction Cell culture Insect Science CD4 Antigens DNA Viral biology.protein HIV-1 Antibody CD81 |
| Zdroj: | Journal of virology. 77(6) |
| ISSN: | 0022-538X |
| Popis: | Macrophages and CD4+lymphocytes are the principal target cells for human immunodeficiency virus type 1 (HIV-1) infection, but the molecular details of infection may differ between these cell types. During studies to identify cellular molecules that could be involved in macrophage infection, we observed inhibition of HIV-1 infection of macrophages by monoclonal antibody (MAb) to the tetraspan transmembrane glycoprotein CD63. Pretreatment of primary macrophages with anti-CD63 MAb, but not MAbs to other macrophage cell surface tetraspanins (CD9, CD81, and CD82), was shown to inhibit infection by several R5 and dualtropic strains, but not by X4 isolates. The block to productive infection was postfusion, as assessed by macrophage cell-cell fusion assays, but was prior to reverse transcription, as determined by quantitative PCR assay for new viral DNA formation. The inhibitory effects of anti-CD63 in primary macrophages could not be explained by changes in the levels of CD4, CCR5, or β-chemokines. Infections of peripheral blood lymphocytes and certain cell lines were unaffected by treatment with anti-CD63, suggesting that the role of CD63 in HIV-1 infection may be specific for macrophages. |
| Databáze: | OpenAIRE |
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