Serotonin increases Na(+)-dependent Ca2+ efflux from bovine adrenal chromaffin cells in culture
| Autor: | Hitoshi Houchi, Masanori Yoshizumi, Kazuo Minakuchi, Motoo Oka, Toshiaki Tamaki, Masumitsu Takasugi, Yasuko Ishimura, Kyoji Morita |
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| Rok vydání: | 1997 |
| Předmět: |
medicine.medical_specialty
Serotonin medicine.drug_class Chromaffin Cells Cyproheptadine Mianserin Internal medicine Adrenal Glands medicine Extracellular Animals Ion transporter Cells Cultured Dose-Response Relationship Drug Chemistry General Neuroscience Osmolar Concentration Sodium Intracellular Membranes Receptor antagonist medicine.anatomical_structure Endocrinology Chromaffin cell Catecholamine Calcium Cattle Efflux Serotonin Antagonists Adrenal medulla Intracellular medicine.drug |
| Zdroj: | Neuroscience letters. 223(1) |
| ISSN: | 0304-3940 |
| Popis: | The effect of serotonin (5-hydroxytryptamine, 5-HT) on Ca2+ mobilization in bovine adrenal chromaffin cells in culture was examined. 5-HT (10−5 M) did not increase secretion of catecholamine, uptake of 45Ca2+ and levels of intracellular free Ca2+ ([Ca2+]i). However, 5-HT (10−8–10−5 M) stimulated the efflux of 45Ca2+ from cultured bovine adrenal chromaffin cells in a concentration-dependent manner. Its stimulatory effect on 45Ca2+ efflux was inhibited by cyproheptadine (a 5-HT1A and 5-HT2 receptor antagonist) or mianserin (a 5-HT2 receptor antagonist). The increase in 5-HT-stimulated 45Ca2+ efflux was dependent on extracellular Na+ concentration, but not extracellular Ca2+ concentration. These results indicate that stimulation of the 5-HT receptors induces extracellular Na+-dependent Ca2+ efflux from bovine adrenal chromaffin cells in culture, probably by acceleration of Na+/Ca2+ exchange. |
| Databáze: | OpenAIRE |
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