Spectrophotometric determination of myoglobin in cardiac and skeletal muscle: Separation from hemoglobin by subunit-exchange chromatography
| Autor: | Jonathan B. Wittenberg, Beatrice A. Wittenberg, Suzie Schuder, Barbara Haseltine |
|---|---|
| Rok vydání: | 1979 |
| Předmět: |
inorganic chemicals
Swine Protein subunit Biophysics Biochemistry Ferrous Birds Sepharose chemistry.chemical_compound Dogs Species Specificity medicine Animals Humans Molecular Biology Skeleton Mammals Chromatography Myoglobin Muscles Myocardium Skeletal muscle Cell Biology Rats medicine.anatomical_structure chemistry biological sciences Cats Fish Cattle Rabbits Hemoglobin Carbon monoxide |
| Zdroj: | Analytical Biochemistry. 92:473-481 |
| ISSN: | 0003-2697 |
| Popis: | Myoglobin is extracted from muscle and separated from blood hemoglobin by subunit-exchange chromatography on a column of Sepharose 4B to which hemoglobin α-β subunits are linked covalently. Hemoglobin is retained on the column. Myoglobin in the effluent is determined spectrophotometrically as ferrous myoglobin or as carbon monoxide ferrous myoglobin. The method is applicable to cardiac, smooth, or skeletal muscle from mammals, reptiles, birds, and teleost fish, but failed with the one amphibian and the one shark tested. |
| Databáze: | OpenAIRE |
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