Screening of endocrine activity of compounds migrating from plastic baby bottles using a multi-receptor panel of in vitro bioassays
| Autor: | Els Van Hoeck, Karin Vandermeiren, Kersten Van Langenhove, Joris Van Loco, Heidi Demaegdt, Adrian Covaci, Marc Elskens, Matthias Onghena, Marie-Louise Scippo, Birgit Mertens, Tara Vandermarken, Coraline Simon |
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| Přispěvatelé: | Chemistry, Earth System Sciences, Analytical, Environmental & Geo-Chemistry |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty medicine.drug_class medicine.medical_treatment Receptors Cytoplasmic and Nuclear 010501 environmental sciences Biology Pharmacology Endocrine Disruptors Toxicology 01 natural sciences Steroid Cell Line 03 medical and health sciences Mice Glucocorticoid receptor Genes Reporter Nuclear receptors Internal medicine Cell Line Tumor medicine Animals Humans Receptor 0105 earth and related environmental sciences Polycarboxylate Cement Pharmacology. Therapy Infant Equipment Endocrine active substances General Medicine Androgen Aryl hydrocarbon receptor Bottle Feeding Reporter gene assay Chemistry 030104 developmental biology Endocrinology Nuclear receptor Endocrine disruptor biology.protein Biological Assay food contact material Hormone |
| Zdroj: | Toxicology in vitro |
| ISSN: | 0887-2333 |
| Popis: | Endocrine activity of 65 compounds migrating from polycarbonate replacement plastic baby bottles was assessed using in vitro cell based assays (reporter gene assays) involving 7 nuclear receptors, i.e. human steroid hormones receptors (oestrogen, androgen, progesterone and glucocorticoid receptors), human thyroid beta and peroxisome proliferator-activated gamma receptors, and the mouse aryl hydrocarbon receptor. The chemicals were tested at 4 concentrations ranging from 0.001 mM to 1 mM. Only twelve chemicals did not show any activity towards any of the nuclear receptors, while fifty three compounds showed a possible endocrine activity. Most of the agonistic activities were observed towards the oestrogen receptor while the PPAR gamma was the target for most of the recorded antagonistic activities. Agonistic activities were recorded for several phthalates, benzophenones, aromatic hydrocarbons and phenols, while compounds such as benzaldehydes, ketones and esters of fatty acid showed antagonistic activities. Thirty five chemicals were able of agonistic activities on 1 to 4 receptors and antagonistic activities were recorded for 35 compounds as well, towards 1 to 7 receptors. Sixteen compounds were able of both agonistic and antagonistic activities, but not on the same receptors, except in 2 cases for the oestrogen receptor and 4 cases for the PPAR gamma. (C) 2016 Elsevier Ltd. All rights reserved. |
| Databáze: | OpenAIRE |
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