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Additional file 1:Expanded and annotated diagram of Figure 2A(early steps in the RAM reaction), and extension of the model for product prediction. Figure S1.1 expands on Figure 3, showing generation of RAM products by primer extension on a template generated by rolling circle amplification. Figure S1.2 illustrates the recursive, or nested, model of RAM products. Tables S1.1, S1.2, and S1.3 extend the table of products concept to generate a prediction of exponential dsDNA product accumulation shown in Figure S1.3. Table S1.4 shows an example of RAM stoichiometry calculation, followed by data and arguments to show that RAM reactions as performed could exhaust the reagent pool. (PDF 245 KB) |
