The Fos protein complex is associated with DNA in isolated nuclei and binds to DNA cellulose
| Autor: | Lidia C. Sambucetti, Tom Curran |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
Regulation of gene expression
Multidisciplinary biology Adrenal Gland Neoplasms DNA Oncogenes Pheochromocytoma Ribonuclease Pancreatic Molecular biology Chromatin Gene product chemistry.chemical_compound Viral Proteins chemistry biology.protein Animals Deoxyribonuclease I Micrococcal Nuclease Electrophoresis Polyacrylamide Gel Ribonuclease Cellulose Polyacrylamide gel electrophoresis Micrococcal nuclease |
| Zdroj: | Science (New York, N.Y.). 234(4782) |
| ISSN: | 0036-8075 |
| Popis: | The properties of the viral and cellular fos proteins (Fos) were investigated as a first step toward understanding the function of the fos gene. Treatment of nuclei with salt and nonionic detergents solubilized a complex that contained Fos together with several other cellular proteins. The majority of the Fos protein complex was released from isolated nuclei incubated in the presence of deoxyribonuclease I or micrococcal nuclease but not with ribonuclease A, suggesting that Fos is associated with chromatin. This hypothesis is supported by the finding that Fos protein from native or denatured nuclear extracts exhibited DNA-binding activity in vitro. These results suggest that Fos is involved in the regulation of gene expression. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|