The RNA Polymerase I Transcription Factor UBF Is the Product of a Primary Response Gene
| Autor: | Dawn E. Larson, Ross D. Hannan, Bruce H. Sells, Laura Taylor, Marija Glibetic, Lawrence I. Rothblum |
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| Rok vydání: | 1995 |
| Předmět: |
Transcription
Genetic General transcription factor Response element RNA polymerase II 3T3 Cells Cell Biology Biology Biochemistry Molecular biology DNA-Binding Proteins Mice Gene Expression Regulation RNA Polymerase I Transcription (biology) biology.protein Animals Transcription factor II F RNA Messenger Transcription factor II D Pol1 Transcription Initiation Complex Proteins Molecular Biology RNA polymerase II holoenzyme Transcription factor II B Transcription Factors |
| Zdroj: | Journal of Biological Chemistry. 270:4209-4212 |
| ISSN: | 0021-9258 |
| Popis: | Transcription of the ribosomal RNA genes by RNA polymerase I is tightly coordinated with the rate of cell growth. The RNA polymerase I transcription factor, UBF, activates transcription by binding to elements within the promoter and enhancer elements within the intergenic spacer but is not required for basal transcription. To assess the role of UBF in modulating ribosomal DNA transcription, we studied its expression in NIH3T6 fibroblasts when transcription was repressed in response to serum starvation and stimulated following refeeding. Our results demonstrate a correlation between the amounts of UBF protein and the rates of ribosomal DNA transcription in quiescent and serum-stimulated cells. Nuclear run-on assays and Northern blot analyses demonstrated that the UBF gene was a primary response gene, exhibiting characteristics similar to those of c-myc and SRF. These results suggest that the regulation of transcription of the UBF gene by polymerase II represents a pathway by which cells modulate transcription by RNA polymerase I. |
| Databáze: | OpenAIRE |
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