Neutrophil-mediated mechanisms of damage and in-vitro protective effect of colchicine in non-vascular Behçet's syndrome
| Autor: | Adalgisa Palermo, Giacomo Emmi, Matteo Becatti, Niccolò Taddei, Alessandra Bettiol, Silvia Galora, Irene Mattioli, Maria Letizia Urban, Amanda Mannucci, Domenico Prisco, Flavia Rita Argento, Eleonora Fini, Claudia Fiorillo, Elena Silvestri, Danilo Malandrino |
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| Rok vydání: | 2021 |
| Předmět: |
Adult
Male Neutrophils Immunology Anti-Inflammatory Agents Inflammation/Inflammatory Disease Pharmacology medicine.disease_cause Extracellular Traps vasculitis Antioxidants Autoimmunity Pathogenesis chemistry.chemical_compound medicine Humans Immunology and Allergy Colchicine human Retrospective Studies reactive oxygen species chemistry.chemical_classification Reactive oxygen species business.industry Behcet Syndrome autoimmunity Neutrophil extracellular traps Middle Aged medicine.disease Oxidative Stress chemistry Apoptosis Case-Control Studies Original Article Female ORIGINAL ARTICLES business Intracellular Systemic vasculitis |
| Zdroj: | Clinical and Experimental Immunology |
| ISSN: | 1365-2249 0009-9104 |
| DOI: | 10.1111/cei.13664 |
| Popis: | Behçet’s syndrome (BS) is a systemic vasculitis with several clinical manifestations. Neutrophil hyperactivation mediates vascular BS pathogenesis, via both a massive reactive oxygen species (ROS) production and neutrophil extracellular traps (NETs) release. Here, we investigated neutrophil‐mediated mechanisms of damage in non‐vascular BS manifestations and explored the in‐vitro effects of colchicine in counteracting these mechanisms. NETs and intracellular ROS production was assessed in blood samples from 80 BS patients (46 with active non‐vascular BS, 34 with inactive disease) and 80 healthy controls. Moreover, isolated neutrophils were incubated for 1 h with an oxidating agent [2,2′‐azobis (2‐amidinopropane) dihydrochloride; 250 nM] and the ability of pure colchicine pretreatment (100 ng/ml) to counteract oxidation‐induced damage was assessed. Patients with active non‐vascular BS showed remarkably increased NET levels [21.2, interquartile range (IQR) = 18.3–25.9 mU/ml] compared to patients with inactive disease (16.8, IQR = 13.3–20.2 mU/ml) and to controls (7.1, IQR = 5.1–8.7 mU/ml, p Neutrophil‐mediated mechanisms of damage might be directly involved in non‐vascular Behçet's syndrome. Neutrophil extracellular traps (NETs), more than an impaired redox status, might play a central role in the pathogenesis of mucosal, articular and intestinal BS manifestations. Colchicine might be effective to counteract neutrophils‐mediated damage in Behçet's syndrome, via the inhibition of NETs release. |
| Databáze: | OpenAIRE |
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