Cudraflavanone B Isolated from the Root Bark of Cudrania tricuspidata Alleviates Lipopolysaccharide-Induced Inflammatory Responses by Downregulating NF-κB and ERK MAPK Signaling Pathways in RAW264.7 Macrophages and BV2 Microglia
| Autor: | Youn-Chul Kim, Nayeon Kim, Tran Hong Quang, Dong-Sung Lee, Eun-Rhan Woo, Hwan Lee, Kwan-Woo Kim, Chi-Su Yoon, Wonmin Ko, Sam-Cheol Kim, Hyuncheol Oh |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Lipopolysaccharides MAP Kinase Signaling System Immunology Down-Regulation Moraceae Nitric oxide 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine Immunology and Allergy Animals Protein kinase A Flavonoids biology Dose-Response Relationship Drug Kinase Macrophages NF-kappa B NF-κB Cell biology Nitric oxide synthase 030104 developmental biology RAW 264.7 Cells chemistry 030220 oncology & carcinogenesis Mitogen-activated protein kinase biology.protein Plant Bark lipids (amino acids peptides and proteins) Tumor necrosis factor alpha Microglia Signal transduction Inflammation Mediators |
| Zdroj: | Inflammation. 44(1) |
| ISSN: | 1573-2576 |
| Popis: | A prenylated flavonoid, cudraflavanone B, is isolated from Cudrania tricuspidata. In this study, we investigated its anti-inflammatory and anti-neuroinflammatory effects in lipopolysaccharide (LPS)-induced RAW264.7 and BV2 cells. In our initial study of the anti-inflammatory effects of cudraflavanone B the production of nitric oxide and prostaglandin E2 was attenuated in LPS-stimulated RAW264.7 and BV2 cells. These inhibitory effects were related to the downregulation of inducible nitric oxide synthase and cyclooxygenase-2. In addition, cudraflavanone B suppressed the production of pro-inflammatory cytokines such as interleukin-6 and tumor necrosis factor-α in LPS-induced RAW264.7 and BV2 cells. Moreover, the evaluation of the molecular mechanisms underlying the anti-inflammatory effects of cudraflavanone B revealed that the compound attenuated the nuclear factor-kappa B signaling pathway in LPS-induced RAW264.7 and BV2 cells. In addition, cudraflavanone B inhibited the phosphorylation of extracellular signal-regulated kinase mitogen-activated protein kinase signaling pathways in these LPS-stimulated cells. Thus, cudraflavanone B suppressed nuclear factor-κB, and extracellular signal-regulated kinase mitogen-activated protein kinase mediated inflammatory pathways, demonstrating its potential in the treatment of neuroinflammatory conditions. |
| Databáze: | OpenAIRE |
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