In Vivo Molecular Imaging of Apoptosisand Necrosis in Atherosclerotic PlaquesUsing MicroSPECT-CT and MicroPET-CT Imaging
| Autor: | Niko Deckers, Felix M. Mottaghy, Dennis H. M. Kusters, Geert Hendrikx, Jan Bucerius, P. Granton, Kim Douma, Chris P. M. Reutelingsperger, Matthias Bauwens, M. De Saint-Hubert, M. Drummen |
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| Přispěvatelé: | RS: NUTRIM - R1 - Metabolic Syndrome, RS: GROW - Oncology, RS: CARIM - R3 - Vascular biology, RS: CARIM - R2 - Cardiac function and failure, RS: GROW - R3 - Innovative Cancer Diagnostics & Therapy, MUMC+: DA BV Medische staf (6), Biochemie, Radiotherapie, Humane Biologie, Beeldvorming |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Cancer Research
Biodistribution Necrosis Apoptosis Single-photon emission computed tomography Multimodal Imaging Mice In vivo medicine Animals Humans Radiology Nuclear Medicine and imaging Tissue Distribution Annexin A5 Perylene Anthracenes Tomography Emission-Computed Single-Photon medicine.diagnostic_test business.industry Technetium Plaque Atherosclerotic Molecular Imaging Oncology Positron emission tomography Positron-Emission Tomography Tomography Molecular imaging medicine.symptom Nuclear medicine business Tomography X-Ray Computed Preclinical imaging |
| Zdroj: | Molecular Imaging and Biology, 16(2), 246-254. Springer |
| ISSN: | 1536-1632 |
| DOI: | 10.1007/s11307-013-0677-0 |
| Popis: | PURPOSE: The purpose of this paper is to study molecular imaging of apoptosis and necrosis, two key players in atherosclerosis instability, using a multimodal imaging approach combining single photon emission computed tomography (SPECT), positron emission tomography (PET), and computed tomography (CT). PROCEDURES: Collar-induced carotid atherosclerosis ApoE knockout mice were imaged with 99mTc-AnxAF568 SPECT-CT to study apoptosis and sequentially with PET-CT following 124I-Hypericin (124I-Hyp) injection to visualize necrosis. RESULTS: SPECT depicted increased 99mTc-AnxAF568 uptake in both atherosclerotic carotid arteries, whereas our data suggest that this uptake is not merely apoptosis related. Although PET of 124I-Hyp was hampered by the slow blood clearance in atherosclerotic mice, 124I-Hyp was able to target necrosis in the atherosclerotic plaque. CONCLUSION: Both 99mTc-AnxAF568 and 124I-Hyp uptake are increased in atherosclerotic carotid vasculature compared to control arteries. While apoptosis imaging remains challenging, necrosis imaging can be feasible after improving the biodistribution characteristics of the probe. |
| Databáze: | OpenAIRE |
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