New reductant-free synthesis of gold nanoparticles-doped chitosan-based semi-IPN nanogel: A robust nanoreactor for exclusively sensitive 5-fluorouracil sensor
| Autor: | Deepak Vishnu S. K, Palraj Ranganathan, Syang-Peng Rwei, Perumal Sarojini, Thavuduraj Kavitha, Cattaleeya Pattamaprom |
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| Rok vydání: | 2020 |
| Předmět: |
Materials science
Polymers Metal Nanoparticles Nanogels Biosensing Techniques 02 engineering and technology Nanoreactor Electrocatalyst Electrochemistry Biochemistry Catalysis Chitosan 03 medical and health sciences chemistry.chemical_compound Limit of Detection Structural Biology Humans Molecular Biology 030304 developmental biology 0303 health sciences General Medicine 021001 nanoscience & nanotechnology chemistry Chemical engineering Methacrylic acid Colloidal gold Fluorouracil Gold 0210 nano-technology Nanogel |
| Zdroj: | International Journal of Biological Macromolecules. 148:79-88 |
| ISSN: | 0141-8130 |
| DOI: | 10.1016/j.ijbiomac.2020.01.108 |
| Popis: | We report a novel class of chitosan (CS)-based hybrid semi-interpenetrating nanogel catalyst by the reductant-free green synthesis of gold nanoparticles (Au NPs) within the CS-poly(methacrylic acid) (Au@CS-PMAA) polymer network. The covalently cross-linked Au@CS-PMAA nanogel with CS chains semi-interpenetrating (semi-IPN) in the PMAA cross-linked network show superior colloidal stability and improved catalytic activity. The synthesized Au@CS-PMAA semi-IPN nanogel was applied as a superior modifier to create a new and sorely selective electrochemical sensing platform for the determination of antileukemic drug 5-fluorouracil (5-FU). The existence of –OH and –COOH groups in the CS and PMAA offers numerous binding sites for 5-FU. This assures the incorporation of Au NPs which affords a larger surface area and ameliorate electrical conductivity. Besides, the synergic effect amid both compounds improves the detection of 5-FU. Under optimal conditions, the constructed sensor offered a good performance in determination of 5-FU with a wide linear range from 0.1–497 μM and low detection limits of 0.03 μM, respectively. Further, this study proved that the fabricated sensor could be useful for analysis of 5-FU in actual human blood serum samples. |
| Databáze: | OpenAIRE |
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