The expression profile of microRNAs in mouse embryos
| Autor: | Sachiko Okamoto, Masahiro Sato, Oleg Mirochnitchenko, Ikunoshin Kato, Junichi Mineno, Hideto Chono, Kiyozo Asada, Masanori Takayama, Tatsuya Ando, Hiroyuki Izu, Masayori Inouye |
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| Rok vydání: | 2006 |
| Předmět: |
Genetics
Regulation of gene expression Mice Inbred BALB C Small RNA Gene Expression Profiling Embryonic Development Gene Expression Regulation Developmental RNA Genomics Computational biology Biology Embryo Mammalian Article Massively parallel signature sequencing Gene expression profiling Mice MicroRNAs Transcription (biology) microRNA Animals Cluster Analysis RNA Small Interfering Gene Library |
| Zdroj: | Nucleic Acids Research |
| ISSN: | 1362-4962 0305-1048 |
| DOI: | 10.1093/nar/gkl096 |
| Popis: | MicroRNAs (miRNAs), which are non-coding RNAs 18-25 nt in length, regulate a variety of biological processes, including vertebrate development. To identify new species of miRNA and to simultaneously obtain a comprehensive quantitative profile of small RNA expression in mouse embryos, we used the massively parallel signature sequencing technology that potentially identifies virtually all of the small RNAs in a sample. This approach allowed us to detect a total of 390 miRNAs, including 195 known miRNAs covering approximately 80% of previously registered mouse miRNAs as well as 195 new miRNAs, which are so far unknown in mouse. Some of these miRNAs showed temporal expression profiles during prenatal development (E9.5, E10.5 and E11.5). Several miRNAs were positioned in polycistron clusters, including one particular large transcription unit consisting of 16 known and 23 new miRNAs. Our results indicate existence of a significant number of new miRNAs expressed at specific stages of mammalian embryonic development and which were not detected by earlier methods. |
| Databáze: | OpenAIRE |
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