Mammalian p53R2 Protein Forms an Active Ribonucleotide Reductasein Vitro with the R1 Protein, Which Is Expressed Both in Resting Cells in Response to DNA Damage and in Proliferating Cells
| Autor: | Astrid Gräslund, Pelle Håkansson, Nina Voevodskaya, Lars Thelander, Hirofumi Arakawa, Olivier Guittet, Susan Fridd, Yusuke Nakamura |
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| Rok vydání: | 2001 |
| Předmět: |
DNA damage
DNA repair Protein subunit Molecular Sequence Data Cell Cycle Proteins In Vitro Techniques Biochemistry law.invention Mice law Ribonucleotide Reductases Animals Humans Amino Acid Sequence RNA Messenger Ribonucleotide Reductase Subunit Cell Cycle Protein Molecular Biology Reporter gene Sequence Homology Amino Acid Chemistry Cell Biology Ribonucleotide reductase Recombinant DNA Electrophoresis Polyacrylamide Gel Cell Division DNA Damage Protein Binding |
| Zdroj: | Journal of Biological Chemistry. 276:40647-40651 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.m106088200 |
| Popis: | Recently, a homologue of the small subunit of mammalian ribonucleotide reductase (RNR) was discovered, called p53R2. Unlike the well characterized S phase-specific RNR R2 protein, the new form was induced in response to DNA damage by the p53 protein. Because the R2 protein is specifically degraded in late mitosis and absent in G0/G1 cells, the induction of the p53R2 protein may explain how resting cells can obtain deoxyribonucleotides for DNA repair. However, no direct demonstration of RNR activity of the p53R2 protein was presented and furthermore, no corresponding RNR large subunit was identified. In this study we show that recombinant, highly purified human and mouse p53R2 proteins contain an iron-tyrosyl free radical center, and both proteins form an active RNR complex with the human and mouse R1 proteins. UV irradiation of serum-starved, G0/G1-enriched mouse fibroblasts, stably transformed with an R1 promoter-luciferase reporter gene construct, caused a 3-fold increase in luciferase activity 24 h after irradiation, paralleled by an increase in the levels of R1 protein. Taken together, our data indicate that the R1 protein can function as the normal partner of the p53R2 protein and that an R1-p53R2 complex can supply resting cells with deoxyribonucleotides for DNA repair. |
| Databáze: | OpenAIRE |
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