Partial Genetic Characterization of Peste Des Petits Ruminants Virus from Goats in Northern and Eastern Tanzania

Autor: Jonas Johansson Wensman, Lughano Kusiluka, Muhammad Munir, Gerald Misinzo, Esron D. Karimuribo, E. S. Macha, Tebogo Kgotlele, J Van Doorsselaere, Christopher J. Kasanga
Rok vydání: 2014
Předmět:
Veterinary medicine
Molecular Sequence Data
Ppr virus
Biology
phylogeny
Tanzania
Peste-des-petits-ruminants virus
Peste-des-Petits-Ruminants
parasitic diseases
medicine
Animals
Cluster Analysis
Base sequence
nucleoprotein gene
DNA Primers
peste des petits ruminants virus
Goat Diseases
Acute viral disease
Base Sequence
Models
Genetic
General Veterinary
General Immunology and Microbiology
Goats
Cutaneous nodules
General Medicine
biology.organism_classification
Virology
peste des petits ruminants
3. Good health
Diarrhea
Nucleoproteins
medicine.symptom
Sequence Alignment
Emerging and Re-Emerging Infectious Diseases in Developing Countries. Guest Editors: Dr Muhammad Munir and Prof Mikael Berg. The Society for Tropical Veterinary Medicine and Wiley Have Published This Supplement With Financial Support From Department of Biomedical Sciences and Veterinary Public Health
Swedish University of Agriculture
Uppsala Sweden
and Avian Viral Diseases Programme
the Pirbright Institute
Uk
Zdroj: Transboundary and Emerging Diseases
ISSN: 1865-1682
1865-1674
DOI: 10.1111/tbed.12229
Popis: Peste des petits ruminants (PPR) is an acute viral disease of small ruminants. The disease was first reported in Tanzania in 2008 when it was confined to the Northern Zone districts bordering Kenya. The present study was carried out to confirm the presence of PPR virus (PPRV) in Tanzania and to establish their phylogenetic relationships. Samples (oculonasal swabs, tissues and whole blood) were obtained from live goats with clinical presentation suggestive of PPR and goats that died naturally in Ngorongoro (Northern Tanzania) and Mvomero (Eastern Tanzania) districts. The clinical signs observed in goats suspected with PPR included fever, dullness, diarrhea, lacrimation, matting of eye lids, purulent oculonasal discharges, cutaneous nodules, erosions on the soft palate and gums and labored breathing. Post mortem findings included pneumonia, congestion of the intestines, and hemorrhages in lymph nodes associated with the respiratory and gastrointestinal systems. PPRV was detected in 21 out of 71 tested animals using primers targeting the nucleoprotein (N) gene. Phylogenetic analysis, based on the N gene, indicated that PPRV obtained from Northern and Eastern Tanzania clustered with PPRV strains of Lineage III, together with PPRV from Sudan and Ethiopia. The findings of this study indicate that there are active PPRV infections in Northern and Eastern Tanzania, suggesting risks for potential spread of PPR in the rest of Tanzania.
Databáze: OpenAIRE
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