Is the ISAC 112 Microarray Useful in the Diagnosis of Pollinosis in Spain?
| Autor: | A Bernard Alonso, C Moya, Laura Sola, F. Feo Brito, Rubén Martínez-Aranguren, Pedro M. Gamboa, Begoña García, Natalia Blanca-López, A Parra, Javier Fernández, Joan Bartra, M D Alonso, Maria L. Sanz, Francisca Gómez, Eloína González-Mancebo, S. Terrados, M I Alvarado, Maria Jose Goikoetxea |
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| Přispěvatelé: | [García,BE, Sola,L] ] Allergology Service, Complejo Hospitalario de Navarra, Pamplona, Spain. [García,BE, Martínez-Aranguren,R, Goikoetxea,MJ, Sanz,ML] IdiSNA, Navarra Institute for Health Research, Spain . [Martínez-Aranguren,R, Alonso, B, Sanz,ML] Allergology and Clinical Immunology Department, Clínica Universidad de Navarra, Pamplona, Spain. [Gamboa,P] Allergology Service, Hospital de Basurto, Bilbao, Spain. [Feo Brito,F] Allergology Service, Hospital General Universitario de Ciudad Real, Ciudad Real, Spain. [Bartra,J] Unitat d’Alèrgia Servei Pneumologia i Alèrgia Respiratoria Universitat de Barcelona, Institut d’Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Centro de Investigaciones Biomédicas en Red de Enfermedades Respiratorias (CIBERES), Barcelona, Spain . [Blanca-López,N] Allergology Service, Hospital Infanta Leonor, Madrid, Spain. [Gómez,F] Allergology Service, IBIMA, Hospital Regional Universitario de Malaga, UMA, Malaga, Spain. [Alvarado,MI] Allergology Service, Hospital Virgen del Puerto, Plasencia, Cáceres, Spain. [Fernández,J] Allergology Service, Hospital General Universitario de Alicante, Alicante, Spain. [Alonso,MD] Allergology Service, Hospital de Alcorcón, Alcorcón, Spain. [Gonzalez-Mancebo,E] Allergology Service, Hospital de Fuenlabrada, Madrid, Spain. [Moya,C] Allergology Service, Complejo Hospitalario Torrecárdenas, Almería, Spain . [Parra,A] Allergology Service, Hospital de A Coruña, A Coruña, Spain. [Terrados,S] Allergology Service, Hospital Universitario Ramon y Cajal, IRYCIS, Madrid, Spain., This research was financially supported by the Carlos III Health Institute (Grant: PI 11/01634), and by grant RD12/0013/00010 from the Spanish Research Network on Adverse Reactions to Allergens and Drugs (RIRAAF, Red de Investigación de Reacciones Adversas a Alérgenos y Fármacos) of the Carlos III Health Institute. |
| Rok vydání: | 2016 |
| Předmět: |
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0301 basic medicine Allergy Microarray Pollination España Gene Expression Named Groups::Persons::Age Groups::Adult::Middle Aged [Medical Subject Headings] medicine.disease_cause Phenomena and Processes::Genetic Phenomena::Genetic Processes::Gene Expression [Medical Subject Headings] Trees Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans [Medical Subject Headings] Profilins Mediana Edad 0302 clinical medicine Alérgenos Grass pollen Especificidad de la Especie Organisms::Eukaryota::Plants::Viridiplantae::Streptophyta::Embryophyta::Angiosperms::Poaceae [Medical Subject Headings] Immunology and Allergy Masculino Accuracy Geographicals::Geographic Locations::Europe::Spain [Medical Subject Headings] Diseases::Immune System Diseases::Hypersensitivity::Hypersensitivity Immediate::Respiratory Hypersensitivity [Medical Subject Headings] Polen Adulto Femenino Organisms::Eukaryota::Plants::Trees [Medical Subject Headings] food and beverages Anatomy::Plant Structures::Plant Components Aerial::Flowering Tops::Flowers::Germ Cells Plant::Pollen [Medical Subject Headings] Profilinas Middle Aged Humanos Analytical Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Epidemiologic Research Design::Sensitivity and Specificity::Predictive Value of Tests [Medical Subject Headings] Inmunoglobulina E Area Under Curve Pollen Hipersensibilidad Respiratoria Female Molecular diagnosis Adult Área Bajo la Curva Expresión Génica Immunology Check Tags::Male [Medical Subject Headings] Analytical Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Microchip Analytical Procedures::Microarray Analysis [Medical Subject Headings] Poaceae 03 medical and health sciences Species Specificity Predictive Value of Tests Árboles Phenomena and Processes::Biological Phenomena::Species Specificity [Medical Subject Headings] Named Groups::Persons::Age Groups::Adult [Medical Subject Headings] Respiratory Hypersensitivity otorhinolaryngologic diseases medicine Humans In patient Chemicals and Drugs::Amino Acids Peptides and Proteins::Proteins::Blood Proteins::Serum Globulins::Immunoglobulins::Antibodies::Immunoglobulin Isotypes::Immunoglobulin E [Medical Subject Headings] Cypress Analytical Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Epidemiologic Research Design::Sensitivity and Specificity::ROC Curve [Medical Subject Headings] Valor Predictivo de las Pruebas business.industry Curva ROC Analytical Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Epidemiologic Methods::Statistics as Topic::Area Under Curve [Medical Subject Headings] Chemicals and Drugs::Amino Acids Peptides and Proteins::Proteins::Cytoskeletal Proteins::Microfilament Proteins::Profilins [Medical Subject Headings] Allergens Immunoglobulin E Microarray Analysis medicine.disease Spanish population 030104 developmental biology Check Tags::Female [Medical Subject Headings] ROC Curve 030228 respiratory system Spain Análisis por Micromatrices Chemicals and Drugs::Biological Factors::Antigens::Allergens [Medical Subject Headings] business Pollen allergy |
| Zdroj: | Journal of Investigational Allergology and Clinical Immunology. 26:92-99 |
| ISSN: | 1018-9068 |
| DOI: | 10.18176/jiaci.0052 |
| Popis: | Journal Article; Multicenter Study; Research Support, Non-U.S. Gov't; BACKGROUND Multiple sensitization is frequent among pollen-allergic patients. The goal of this study was to determine the diagnostic accuracy of the ImmunoCAP ISAC 112 (ISAC112) microarray in allergy to pollen from several taxa and its clinical utility in a Spanish population. METHODS Specific IgE was determined in 390 pollen-allergic patients using the ISAC112 microarray. Diagnostic accuracy (sensitivity, specificity, predictive values, and area under the ROC curve) was calculated for the diagnosis of allergy to pollen from grass (n=49), cypress (n=75), olive tree (n=33), plane tree (n=63), and pellitory of the wall (n=17) and compared with that of the singleplex ImmunoCAP immunoassay. RESULTS The sensitivity of the ISAC112 microarray ranged from 68.2% for allergy to plane tree pollen to 93.9% for allergy to grass pollen. The specificity was >90%. The AUC for the diagnosis of allergy to plane tree pollen was 0.798, whereas the AUC for the remaining cases was ≥0.876. The accuracy of ISAC112 was higher than that of ImmunoCAP for plane tree pollen and similar for the remaining pollens. The frequency of sensitization to most species-specific allergenic components and profilins varied between the different geographical regions studied. A total of 73% of pollen-allergic patients were sensitized to species-specific components of more than 1 pollen type. CONCLUSIONS The ISAC112 microarray is an accurate tool for the diagnosis of allergy to pollen from grass, cypress, olive tree, plane tree, and pellitory of the wall. The features of the ISAC112 microarray are similar or superior (in the case of plane tree pollen) to those of ImmunoCAP. This microarray is particularly useful for the etiologic diagnosis of pollinosis in patients sensitized to multiple pollen species whose pollination periods overlap. Yes |
| Databáze: | OpenAIRE |
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