Intrasectoral Zoning of Proteins and Nucleotides in Simple Crystalline Hosts

Autor:	Daniel Fredrickson, Werner Kaminsky, Christine A. Mitchell, Bart Kahr, Loyd D. Bastin, Miki Kurimoto, Pamela N. Gustafson, Scott Lovell, Jean Chmielewski, Sei-Hum Jang
Rok vydání:	2000
Předmět:	chemistry.chemical_classification Materials science Oligonucleotide Stereochemistry Matrix isolation Model system Phosphate chemistry.chemical_compound Crystallography chemistry Nucleotide Luminescence Single crystal Vicinal
Zdroj:	Scopus-Elsevier
ISSN:	1946-4274 0272-9172
DOI:	10.1557/proc-620-m9.8.1
Popis:	Oriented gases of biopolymers in simple, single crystal hosts might be used to measure anisotropic molecular properties of analytes that could not otherwise be crystallized. Here we show two types of crystals as examples of the single crystal matrix isolation of biopolymers: green fluorescent protein in α-lactose monohydrate as a model system for studying the kinetic stabilization of biopharmaceuticals, and adenosine phosphates in potassium dihydrogen phosphate, a first step in the matrix isolation of oligonucleotides. In each case, the hosts undergo compositional zoning – both intersectoral and intrasectoral – during growth from solution. Intrasectoral zoning is evident by the selective luminescence of adjacent vicinal slopes of growth active hillocks. Nucleotides furthermore distinguish between symmetry related growth sectors enantioselectively.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::469c2727d7f569a14614e3ac9ca633b6 https://doi.org/10.1557/proc-620-m9.8.1 Zobrazit plný text záznamu Full text from SpringerLink