Penicillin acylase purification with the aid of pseudo-affinity chromatography

Autor:	Nicole Verdoni, Xavier Santarelli, V. Fitton, Jean Sanchez
Rok vydání:	2001
Předmět:	Chromatography Chemistry Ligand Ion chromatography Biophysics Enzymes Immobilized Ligands Biochemistry Chromatography Affinity Penicillin Acylase Affinity chromatography Metal affinity chromatography Protein purification Penicillin Amidase
Zdroj:	Journal of biochemical and biophysical methods. 49(1-3)
ISSN:	0165-022X
Popis:	The introduction of affinity chromatography has opened a new dimension in protein purification. This article reviews the current techniques used in the penicillin acylase purification process, especially pseudo-affinity chromatography. A profile for a suitable ligand is established. An aromatic ring and the presence of one or several amino groups seem essential for proper interaction. Immobilized metal affinity chromatography now seems to be a good competitor.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::469aa36f63432257b02c51a34ddd94db https://pubmed.ncbi.nlm.nih.gov/11694301 Zobrazit plný text záznamu Full Text from ScienceDirect