Temporary effects of neonatal overfeeding on homeostatic control of food intake involve alterations in POMC promoter methylation in male rats
Autor: | Maria Florencia Andreoli, Guillermina Canesini, Gisela Paola Lazzarino, Ana Paula García, Rocio Schumacher, Cora Stoker, Jorge G. Ramos, María Florencia Rossetti |
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Rok vydání: | 2021 |
Předmět: |
Male
0301 basic medicine endocrine system medicine.medical_specialty Pro-Opiomelanocortin Time Factors 030209 endocrinology & metabolism Biology Biochemistry Cocaine and amphetamine regulated transcript 03 medical and health sciences 0302 clinical medicine Endocrinology Internal medicine medicine Animals Homeostasis Weaning RNA Messenger Rats Wistar Promoter Regions Genetic Receptor Molecular Biology Epididymis Leptin Body Weight Neuropeptides digestive oral and skin physiology Arcuate Nucleus of Hypothalamus Gene Expression Regulation Developmental Feeding Behavior DNA Methylation Neuropeptide Y receptor 030104 developmental biology Adipose Tissue Animals Newborn DNA methylation Metabolome Developmental plasticity CpG Islands Ghrelin hormones hormone substitutes and hormone antagonists |
Zdroj: | Molecular and Cellular Endocrinology. 522:111123 |
ISSN: | 0303-7207 |
DOI: | 10.1016/j.mce.2020.111123 |
Popis: | A small litter (SL) model was used to determine how neonatal overfeeding affects the homeostatic control of food intake in male rats at weaning and postnatal day (PND) 90. At PND4, litters were reduced to small (4 pups/dam) or normal (10 pups/dam) litters. At weaning, SL rats showed higher body weight and characteristic features of the metabolic syndrome. Gene expression of pro-opiomelanocortin (POMC), cocaine and amphetamine regulated transcript, neuropeptide Y (NPY) and leptin and ghrelin (GHSR) receptors were increased and POMC promoter was hypomethylated in arcuate nucleus, indicating that the early development of obesity may involve the GHSR/NPY system and changes in POMC methylation state. At PND90, body weight, metabolic parameters and gene expression were restored; however, POMC methylation state remained altered. This work provides insight into the effects of neonatal overfeeding, showing the importance of developmental plasticity in restoring early changes in central pathways involved in metabolic programming. |
Databáze: | OpenAIRE |
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