Signal-to-background ratio and lateral resolution in deep tissue imaging by optical coherence microscopy in the 1700 nm spectral band
| Autor: | Masahito Yamanaka, Norihiko Nishizawa, Naoki Hayakawa |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Materials science Image quality lcsh:Medicine Lateral resolution 01 natural sciences Signal Article Optical imaging 010309 optics 03 medical and health sciences Optics 0103 physical sciences lcsh:Science Multidisciplinary Test target business.industry Resolution (electron density) lcsh:R Imaging and sensing Deep tissue imaging Spectral bands Confocal microscopy 030104 developmental biology Optical coherence microscopy lcsh:Q business |
| Zdroj: | Scientific Reports, Vol 9, Iss 1, Pp 1-8 (2019) Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-019-52175-9 |
| Popis: | We quantitatively investigated the image quality in deep tissue imaging with optical coherence microscopy (OCM) in the 1700 nm spectral band, in terms of the signal-to-background ratio (SBR) and lateral resolution. In this work, to demonstrate the benefits of using the 1700 nm spectral band for OCM imaging of brain samples, we compared the imaging quality of OCM en-face images obtained at the same position by using a hybrid 1300 nm/1700 nm spectral domain (SD) OCM system with shared sample and reference arms. By observing a reflective resolution test target through a 1.5 mm-thick tissue phantom, which had a similar scattering coefficient to brain cortex tissue, we confirmed that 1700 nm OCM achieved an SBR about 6-times higher than 1300 nm OCM, although the lateral resolution of the both OCMs was similarly degraded with the increase of the imaging depth. Finally, we also demonstrated high-contrast deep tissue imaging of a mouse brain at a depth up to 1.8 mm by using high-resolution 1700 nm SD-OCM. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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