Burkholderia species associated with legumes of Chiapas, Mexico, exhibit stress tolerance and growth in aromatic compounds
| Autor: | Arnoldo Wong-Villarreal, Gustavo Yañez-Ocampo, José A. de León-Martínez |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Microbiology (medical) DNA Bacterial Burkholderia 030106 microbiology Microbiology Burkholderia phenoliruptrix 03 medical and health sciences Mimosa pigra Stress Physiological RNA Ribosomal 16S Botany Symbiosis Mimosa diplotricha Mexico Phylogeny biology Mimosa pudica Burkholderia tuberum food and beverages Fabaceae General Medicine Sequence Analysis DNA bacterial infections and mycoses biology.organism_classification Indigofera bacteria Burkholderia phymatum |
| Zdroj: | Revista Argentina de microbiologia. 49(4) |
| ISSN: | 0325-7541 |
| Popis: | Leguminous plants have received special interest for the diversity of β-proteobacteria in their nodules and are promising candidates for biotechnological applications. In this study, 15 bacterial strains were isolated from the nodules of the following legumes: Indigofera thibaudiana, Mimosa diplotricha, Mimosa albida, Mimosa pigra, and Mimosa pudica, collected in 9 areas of Chiapas, Mexico. The strains were grouped into four profiles of genomic fingerprints through BOX-PCR and identified based on their morphology, API 20NE biochemical tests, sequencing of the 16S rRNA, nifH and nodC genes as bacteria of the Burkholderia genus, genetically related to Burkholderia phenoliruptrix, Burkholderia phymatum, Burkholderia sabiae, and Burkholderia tuberum. The Burkholderia strains were grown under stress conditions with 4% NaCl, 45°C, and benzene presence at 0.1% as the sole carbon source. This is the first report on the isolation of these nodulating species of the Burkholderia genus in legumes in Mexico. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|