| Autor: |
Sisi Fan, Paul S. Freemont, Bower I, J Pilcher, Wenqiang Chi B, Richard I. Kitney, Ho Wai Chin M, Andrew I. Webb, J Strutt, Richard Kelwick, Kirsten Jensen, M. Kopniczky, Guy-Bart Stan |
| Jazyk: |
angličtina |
| Rok vydání: |
2014 |
| Předmět: |
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| DOI: |
10.1101/006106 |
| Popis: |
Biopolymers, such as poly-3-hydroxy-butyrate (P(3HB)) are produced as a carbon store in an array of organisms and exhibit characteristics which are similar to oil-derived plastics, yet have the added advantages of biodegradability and biocompatibility. Despite these advantages, P(3HB) production is currently more expensive than the production of oil-derived plastics, and therefore more efficient P(3HB) production processes are required. In this study, we describe the model-guided design and experimental characterization of several engineered P(3HB) producing operons. In particular, we describe the characterization of a novel hybrid phaCAB operon that consists of a dual promoter (native and J23104) and RBS (native and B0034) design. P(3HB) production was around six-fold higher in hybrid phaCAB engineered Escherichia coli in comparison to E. coli engineered with the native phaCAB operon from Ralstonia eutropha H16. The hybrid phaCAB operon represents a step towards the more efficient production of P(3HB), which has an array of applications from 3D printing to tissue engineering. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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