Synergistic Effects of Bone Mesenchymal Stem Cells and Chondroitinase ABC on Nerve Regeneration After Acellular Nerve Allograft in Rats
| Autor: | Hua Jia, Ying Wang, Gui-Bo Liu, Xiao-Jie Tong, Wen-Yuan Li, Si-Wen Kang |
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| Rok vydání: | 2011 |
| Předmět: |
Male
medicine.medical_specialty Pathology Nerve guidance conduit Chondroitin ABC Lyase Mesenchymal Stem Cell Transplantation Nerve conduction velocity Rats Sprague-Dawley Random Allocation Cellular and Molecular Neuroscience medicine Animals Transplantation Homologous Nerve Tissue Rats Wistar Axon Cells Cultured Bone Marrow Transplantation Nerve allograft business.industry Regeneration (biology) Cell Biology General Medicine Nerve Regeneration Rats Surgery Transplantation medicine.anatomical_structure Peripheral nerve injury Female Sciatic nerve Sciatic Neuropathy business |
| Zdroj: | Cellular and Molecular Neurobiology. 32:361-371 |
| ISSN: | 1573-6830 0272-4340 |
| Popis: | This study aimed to evaluate whether combination therapy of bone marrow stromal cells (BMSCs) transplantation and chondroitinase ABC (ChABC) treatment further enhances axonal regeneration and functional recovery after acellular nerve allograft repair of the sciatic nerve gap in rats. Eight Sprague-Dawley rats were used as nerve donors, and 32 Wistar rats were randomly divided into four groups: Group I: acellular rat sciatic nerve (ARSN) group; Group II: ChABC treatment; Group III: BMSCs transplantation; and Group IV: ChABC treatment and BMSCs transplantation. The results showed that compared with ARSN control group, BMSC transplantation promoted axonal regeneration, the secretion of neural trophic factors NGF, BDNF and axon angiogenesis in nerve graft. ChABC treatment degraded chondroitin sulfate proteoglycans in ARSN in vitro and in vivo and improved BMSCs survival in ARSN. The combination therapy caused much better beneficial effects evidenced by increasing sciatic function index, nerve conduction velocity, restoration rate of tibialis anterior wet muscle weight, and myelinated nerve number, but did not further boost the therapeutic effects on neurotrophic factor production, axon angiogenesis, and sensory functional recovery by BMSC transplantation. Taken together, for the first time, we demonstrate the synergistic effects of BMSC transplantation and BMSCs treatment on peripheral nerve regeneration, and our findings may help establish novel strategies for cell transplantation therapy for peripheral nerve injury. |
| Databáze: | OpenAIRE |
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