EPSPS variability, gene expression, and enzymatic activity in glyphosate-resistant biotypes of Digitaria insularis
| Autor: | R. Victoria Filho, Helaine Carrer, Tarcísio Sales Vasconcelos, A. J. P. Albrecht, Andrés López-Rubio, Esteban Galeano, A. A. M. Barroso |
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| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
Population Glycine Gene Expression 01 natural sciences Complementary DNA Gene expression Genetics Proline Genetic variability Allele education Molecular Biology Phylogeny Plant Proteins education.field_of_study Polymorphism Genetic biology Herbicides 04 agricultural and veterinary sciences General Medicine Sequence Analysis DNA biology.organism_classification Molecular biology PLANTAS INVASORAS Amino Acid Substitution Digitaria 040103 agronomy & agriculture 0401 agriculture forestry and fisheries 3-Phosphoshikimate 1-Carboxyvinyltransferase Weed Digitaria insularis 010606 plant biology & botany Herbicide Resistance |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1676-5680 |
| Popis: | Weed resistance to herbicides is a natural phenomenon that exerts selection on individuals in a population. In Brazil, glyphosate resistance was recently detected in Digitaria insularis. The objective of this study was to elucidate mechanisms of weed resistance in this plant, including genetic variability, allelism, amino acid substitutions, gene expression, and enzymatic activity levels. Most of these have not previously been studied in this species. D. insularis DNA sequences were used to analyze genetic variability. cDNA from resistant and susceptible plants was used to identify mutations, alleles, and 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) expression, using real-time quantitative reverse transcription-polymerase chain reaction. In addition, EPSPS activity was measured. We found a decrease in genetic variability between populations related to glyphosate application. Substitutions from proline to threonine and tyrosine to cysteine led to a decrease in EPSPS affinity for the glyphosate. In addition, the EPSPS enzymatic activity was slightly higher in resistant plants, whereas EPSPS gene expression was almost identical in both biotypes, suggesting feedback regulation at different levels. To conclude, our results suggest new molecular mechanisms used by D. insularis to increase glyphosate resistance. |
| Databáze: | OpenAIRE |
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