Transcriptome profile of Corynebacterium pseudotuberculosis in response to iron limitation
| Autor: | Anne Cybelle Pinto Gomide, Alice R. Wattam, Aristóteles Góes-Neto, Thiago Luiz de Paula Castro, Preetam Ghosh, Emannuel Maltempi Souza, Vasco Azevedo, Debmalya Barh, Mariana Teixeira Dornelles Parise, Doglas Parise, Michelle Zibetti Tadra Sfeir, Izabela Coimbra Ibraim |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Genomic Islands Transcription Genetic lcsh:QH426-470 Operon Corynebacterium pseudotuberculosis lcsh:Biotechnology Mutant Respiratory chain Biology 01 natural sciences Transcriptome 03 medical and health sciences chemistry.chemical_compound Iron homeostasis lcsh:TP248.13-248.65 Genetics Gene Regulatory Networks Gene Differential gene expression 030304 developmental biology 0303 health sciences Microbial Viability Iron-regulated transcriptional factors Gene Expression Profiling Iron Deficiencies lcsh:Genetics Heme acquisition chemistry Mutation Transposon mutagenesis Research Article 010606 plant biology & botany Biotechnology Hemin |
| Zdroj: | BMC Genomics, Vol 20, Iss 1, Pp 1-24 (2019) BMC Genomics |
| ISSN: | 1471-2164 |
| Popis: | Background Iron is an essential micronutrient for the growth and development of virtually all living organisms, playing a pivotal role in the proliferative capability of many bacterial pathogens. The impact that the bioavailability of iron has on the transcriptional response of bacterial species in the CMNR group has been widely reported for some members of the group, but it hasn’t yet been as deeply explored in Corynebacterium pseudotuberculosis. Here we describe for the first time a comprehensive RNA-seq whole transcriptome analysis of the T1 wild-type and the Cp13 mutant strains of C. pseudotuberculosis under iron restriction. The Cp13 mutant strain was generated by transposition mutagenesis of the ciuA gene, which encodes a surface siderophore-binding protein involved in the acquisition of iron. Iron-regulated acquisition systems are crucial for the pathogenesis of bacteria and are relevant targets to the design of new effective therapeutic approaches. Results Transcriptome analyses showed differential expression in 77 genes within the wild-type parental T1 strain and 59 genes in Cp13 mutant under iron restriction. Twenty-five of these genes had similar expression patterns in both strains, including up-regulated genes homologous to the hemin uptake hmu locus and two distinct operons encoding proteins structurally like hemin and Hb-binding surface proteins of C. diphtheriae, which were remarkably expressed at higher levels in the Cp13 mutant than in the T1 wild-type strain. These hemin transport protein genes were found to be located within genomic islands associated with known virulent factors. Down-regulated genes encoding iron and heme-containing components of the respiratory chain (including ctaCEF and qcrCAB genes) and up-regulated known iron/DtxR-regulated transcription factors, namely ripA and hrrA, were also identified differentially expressed in both strains under iron restriction. Conclusion Based on our results, it can be deduced that the transcriptional response of C. pseudotuberculosis under iron restriction involves the control of intracellular utilization of iron and the up-regulation of hemin acquisition systems. These findings provide a comprehensive analysis of the transcriptional response of C. pseudotuberculosis, adding important understanding of the gene regulatory adaptation of this pathogen and revealing target genes that can aid the development of effective therapeutic strategies against this important pathogen. Electronic supplementary material The online version of this article (10.1186/s12864-019-6018-1) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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