Redox response, antibacterial and drug package capacities of chitosan-α-lipoic acid conjugates
| Autor: | Qiang Luo, Yanqing Wang, Lixiang Chen, Qianqian Han, Xinran Fan, Hongmei Zhang, Zhenghao Fei, Ying Wang |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Thermogravimetric analysis
Reducing agent 02 engineering and technology Biochemistry Chitosan Mice 03 medical and health sciences chemistry.chemical_compound Structural Biology Cell Line Tumor Escherichia coli Copolymer Animals Fourier transform infrared spectroscopy Molecular Biology 030304 developmental biology Drug Carriers 0303 health sciences Thioctic Acid Hydrogels General Medicine Apparent viscosity 021001 nanoscience & nanotechnology Anti-Bacterial Agents Methotrexate chemistry Polymerization 0210 nano-technology Antibacterial activity Nuclear chemistry |
| Zdroj: | International Journal of Biological Macromolecules. 154:1166-1174 |
| ISSN: | 0141-8130 |
| DOI: | 10.1016/j.ijbiomac.2019.10.271 |
| Popis: | Chitosan-α-lipoic acid (CS-LA) conjugates were prepared by amino reaction. The methotrexate (MTX) was successfully loaded within CS-LA copolymer by the ring-opening polymerizations of disulfide bond under the action of reducing agent. The obtained copolymer was investigated by fourier transform infrared spectrometer (FTIR), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and nuclear magnetic resonance (1H NMR) analysis. The ring-opening polymerization process effected on rheological properties were studied. The graft of LA increased the apparent viscosity of CS-LA, and the loaded of MTX by redox reduced its apparent viscosity enormously. The load of MTX under the action of DTT decreased the apparent viscosity and moduli of CS-LA-MTX hydrogel. In dynamic oscillatory experiment, the package of MTX reduced the rigidity of CS-LA and damaged the gel structure of CS-LA. The antimicrobial ability of CS-LA against E. coli was processed by the inhibition zone and growth curves. The modification of LA raised the antibacterial activity of CS-LA. The cytotoxicity assay of CS-LA-MTX to prostate cancer cells (RM-1) was examined. The load of MTX within CS-LA-MTX greatly enhanced the cytotoxicity to RM-1 cells. In summary, CS-LA hydrogel may provide a new copolymer carrier for drug package in food and biomedical application. |
| Databáze: | OpenAIRE |
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