FOXP3 variants are independently associated with transforming growth factor Β1 plasma levels in female patients with inflammatory bowel disease
| Autor: | Cláudia Junko Inoue, Tamires Flauzino, Beatriz Piantoni Gonçalves, Jaqueline Costa Castardo de Paula, Talita Cristina Galvão, Paula Kikuchi Miyazaki, Camila Cataldi de Alcantara, Lucilene Rosa e Silva Westmore, Marcell Alysson Batisti Lozovoy, Edna Maria Vissoci Reiche, Andréa Name Colado Simão |
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| Rok vydání: | 2022 |
| Předmět: |
Transforming growth Factor β1
Forkhead Transcription Factors General Medicine Inflammatory bowel diseases Polymorphism Single Nucleotide Interleukin-10 Transforming Growth Factor beta1 Crohn's disease Forkhead box protein 3 genetic variants Ulcerative colitis Crohn Disease Humans Colitis Ulcerative Female Genetic Predisposition to Disease |
| Zdroj: | Clinics, Volume: 77, Article number: 100084, Published: 09 SEP 2022 |
| ISSN: | 1807-5932 |
| Popis: | Objective: The aim of this study was to evaluate the association of -924 G>A (rs2232365) and -3279 C>A (rs3761548) FOXP3 variants with IBD susceptibility, clinical and endoscopic activity, and IL-10 and TGF-β1 plasma levels. Method: The study included 110 IBD female patients, 60 with Ulcerative Colitis (UC) and 50 with Crohn’s Disease (CD), and 154 female controls. FOXP3 variants were determined with Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP). Plasma levels of IL-10 and TGF-β1 were determined using immuno-fluorimetric assay. Results: AA genotype of rs2232365 and rs3761548 was associated with CD (OR = 3.147, 95% CI 1.015-9.758, p = 0.047) and UC (OR = 3.221, 95% CI 1.050-9.876, p = 0.041) susceptibility, respectively. However, were not associated with TGF-β1 and IL-10 levels, and endoscopic/clinical activity disease. GAGA haplotype was associated with IBD (OR = 4.003, 95% CI 1.100–14.56, p = 0.035) and UC susceptibility (OR = 6.107, 95% CI 1.609–23.18, p = 0.008). In addition, IBD patients with the GAGA haplotype had lower TGF-β1 levels (p = 0.041). Moreover, G/C haplotype (dominant model) had a protective effect of 60% in CD susceptibility and lower Endoscopic Severity Index. Conclusions: These results suggest that FOXP3 variants could exert a role in the Treg, which could be one of the factors involved in the susceptibility and pathogenesis of IBD. |
| Databáze: | OpenAIRE |
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