Whole genomic sequencing based genotyping reveals a specific X3 sublineage restricted to Mexico and related with multidrug resistance
| Autor: | Cuauhtemoc Licona-Cassani, Roberto Zenteno-Cuevas, Paulina M. Mejía-Ponce, Irving Cancino-Muñoz, Carlos Francisco Madrazo-Moya, Ana Cristina Jiménez-Ruano, Raquel Muñiz-Salazar, Iñaki Comas |
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| Přispěvatelé: | Universidad Veracruzana, Comas, Iñaki [0000-0001-5504-9408], Comas, Iñaki |
| Rok vydání: | 2021 |
| Předmět: |
Adult
DNA Bacterial Male 0301 basic medicine Tuberculosis Genotype Genotyping Techniques Molecular biology Science Antitubercular Agents Biology Microbiology Polymorphism Single Nucleotide Article 03 medical and health sciences 0302 clinical medicine Drug Resistance Multiple Bacterial Tuberculosis Multidrug-Resistant medicine Humans 030212 general & internal medicine Mexico Genotyping Phylogeny Whole genome sequencing Genetics Multidisciplinary High prevalence Whole Genome Sequencing Drug resistant tuberculosis Genomic sequencing Genomics Mycobacterium tuberculosis Middle Aged medicine.disease Multiple drug resistance Cross-Sectional Studies 030104 developmental biology Multigene Family Medicine Female |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname Scientific Reports, Vol 11, Iss 1, Pp 1-9 (2021) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | 9 páginas, 1 figurra, 3 tablas. Te online version contains supplementary material available at https://doi.org/10.1038/s41598-020-80919-5. Whole genome sequencing (WGS) has been shown to be superior to traditional procedures of genotyping in tuberculosis (TB), nevertheless, reports of its use in drug resistant TB (DR-TB) isolates circulating in Mexico, are practically unknown. Considering the above the main of this work was to identify and characterize the lineages and genomic transmission clusters present in 67 DR-TB isolates circulating in southeastern Mexico. The results show the presence of three major lineages: L1 (3%), L2 (3%) and L4 (94%), the last one included 16 sublineages. Sublineage 4.1.1.3 (X3) was predominant in 18 (27%) of the isolates, including one genomic cluster, formed by eleven multidrug resistant isolates and sharing the SIT 3278, which seems to be restricted to Mexico. By the use of WGS, it was possible to identify the high prevalence of L4 and a high number of sublineages circulating in the region, also was recognized the presence of a novel X3 sublineage, formed exclusively by multidrug resistant isolates and with restrictive circulation in Mexico for at least the past 17 years. AC-JR and CFM-M were CONACYT fellows of the Health Sciences Master of the Health Sciences Institute of Universidad Veracruzana. RZ-C and IC were funded by i-COOP-20017-COOPB2032. |
| Databáze: | OpenAIRE |
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