Senescence and adiponectin signaling : studies in canine testis
| Autor: | Wiktor Turek, Malgorzata Kotula-Balak, Agnieszka Rak, Anna Galuszka, Agnieszka Pietsch-Fulbiszewska, Kazimierz Tarasiuk, Grzegorz Ramisz, Maria Chmurska-Gasowska |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
0301 basic medicine Senescence medicine.medical_specialty senescence Biology testis 03 medical and health sciences Dogs Internal medicine Cryptorchidism Testis medicine Extracellular Animals Testosterone Adiponectin receptor 1 Adiponectin adiponectin General Medicine 030104 developmental biology medicine.anatomical_structure Endocrinology dog Immunohistochemistry 030101 anatomy & morphology Anatomy Signal transduction cryptorchidism Germ cell Signal Transduction Developmental Biology |
| Popis: | Background The meaning of senescence for tissue physiological and pathological conditions is poorly known. Based on initial reports especially proteins and mechanisms that regulate this process are necessary to be determinate. Methods The main aim of the study was to investigate the presence of senescent cells in canine testicular tissue (mixed breed testes; n = 60) in relation to adiponectin signaling. In detail, new information on the senescence cell number, as well as senescence and adiponectin signaling mechanisms in cryptorchid and germ cell tumor testes were provided with the use of immunohistochemical and colorimetric analyses. Results Comparison of immunohistochemical results, in cryptorchid and tumor testes revealed increased number of senescent cells (p16 and γH2AX markers). Increased expression of adiponectin and adiponectin receptor 1, as well as extracellular signal-activated kinase (ERK1/2) in pathological testes were detected. In addition, decreased cholesterol and increased testosterone levels in tumor testis were found. Conclusion The present study is the first to demonstrate the presence as well as the differences that exist in senecent cell number in mixed breed dog testes with cryptorchidism and germ cell tumor. Altered expression of adiponectin signaling and ERK1/2 signaling pathways together with altered cholesterol and testosterone levels reflect important senescence role in disturbed functions of canine testis. Moreover, the application of studied here senescence regulating molecules for detection and prevention against pathologies of the male gonad should be furtherly considered. |
| Databáze: | OpenAIRE |
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