Tegillarca granosa extract Haishengsu (HSS) suppresses expression of mdr1, BCR/ABL and sorcin in drug-resistant K562/ADM tumors in mice
| Autor: | Bin Zhang, M. Hou, SG Chen, JZ Liu, Lexin Wang, GY Li, M. Yang |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Combination therapy
Fusion Proteins bcr-abl Mice Nude Apoptosis Flow cytometry Mice hemic and lymphatic diseases Albumins Antineoplastic Combined Chemotherapy Protocols medicine Animals Humans ATP Binding Cassette Transporter Subfamily B Member 1 Tegillarca granosa ABL biology medicine.diagnostic_test Dose-Response Relationship Drug Caspase 3 Gene Expression Profiling Calcium-Binding Proteins breakpoint cluster region Cancer General Medicine biology.organism_classification medicine.disease Flow Cytometry Drug Resistance Multiple Bivalvia Doxorubicin Drug Resistance Neoplasm Cancer research Female K562 Cells Neoplasm Transplantation K562 cells Drugs Chinese Herbal |
| Zdroj: | Advances in medical sciences. 58(1) |
| ISSN: | 1898-4002 |
| Popis: | To evaluate the effect of Haishengsu (HSS), a protein extract from Tegillarca granosa, on multidrug-resistance genes mdr1, BCR/ABL and sorcin in transplanted tumors.Mice were inoculated subcutaneously with a drug resistant leukemia cell line K562/ADM. Tumor-bearing animals were divided into control, adriamycin, HSS and combination therapy (adriamycin plus HSS) groups. Flow cytometry was used to detect apoptosis of tumor cells, and RT-PCR was used to evaluate the expression of mdr1, BCR/ABL and sorcin.The apoptosis rate in the high (71.8%), medium (72.3%) and low doses HSS group (72.4%) was higher than in control (1.2%, p0.01), adriamycin (34.4%, p0.05) or combination therapy group (46.4%, p0.05). The mean optical density of mdr1, BCR/ABL and sorcin in HSS groups was lower than in control, adriamycin and combination therapy group (p0.01). The optical density of the three genes in high HSS group was lower than in medium and low HSS group (p0.01).Haishengsu promotes apoptosis of drug-resistant K562/ADM tumors in mice in a dose-dependent manner. The pro-apoptotic effect of Haishengsu may be related to a reduced expression of multidrug-resistance genes mdr1, BCR/ABL and sorcin. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect