A facile and selective approach for enrichment of l-cysteine in human plasma sample based on zinc organic polymer: Optimization by response surface methodology
| Autor: | Abbas Ostovan, Hamedreza Javadian, Sonia Bahrani, Mehrorang Ghaedi, Tahere Taghipour, Mohammad Javad Khoshnood Mansoorkhani |
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| Rok vydání: | 2017 |
| Předmět: |
Aging
Calibration curve Surface Properties Clinical Biochemistry Pharmaceutical Science chemistry.chemical_element 02 engineering and technology Zinc 01 natural sciences High-performance liquid chromatography Analytical Chemistry Adsorption X-Ray Diffraction Limit of Detection Drug Discovery Spectroscopy Fourier Transform Infrared Humans Response surface methodology Cysteine Spectroscopy Chromatography High Pressure Liquid Metal-Organic Frameworks Solid Phase Microextraction Detection limit Analysis of Variance Chromatography 010401 analytical chemistry Extraction (chemistry) 021001 nanoscience & nanotechnology 0104 chemical sciences chemistry Ultrasonic Waves Microscopy Electron Scanning Feasibility Studies 0210 nano-technology Powder Diffraction |
| Zdroj: | Journal of pharmaceutical and biomedical analysis. 149 |
| ISSN: | 1873-264X |
| Popis: | In this research, a facile and selective method was described to extract l -cysteine ( l -Cys), an essential α-amino acid for anti-ageing playing an important role in human health, from human blood plasma sample. The importance of this research was the mild and time-consuming synthesis of zinc organic polymer (Zn-MOP) as an adsorbent and evaluation of its ability for efficient enrichment of l -Cys by ultrasound-assisted dispersive micro solid-phase extraction (UA-DMSPE) method. The structure of Zn-MOP was investigated by FT-IR, XRD and SEM. Analysis of variance (ANOVA) was applied for the experimental data to reach the best optimum conditions. The quantification of l -Cys was carried out by high performance liquid chromatography with UV detection set at λ = 230 nm. The calibration graph showed reasonable linear responses towards l -Cys concentrations in the range of 4.0–1000 μg/L (r 2 = 0.999) with low limit of detection (0.76 μg/L, S/N = 3) and RSD ≤ 2.18 (n = 3). The results revealed the applicability and high performance of this novel strategy in detecting trace l -Cys by Zn-MOP in complicated matrices. |
| Databáze: | OpenAIRE |
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