Erythrocyte nitric oxide availability and oxidative stress following exercise
| Autor: | Raul Almeira Freire, Marcela Anjos Martins, Wanda Vianna Mury, Walace David Monteiro, Antônio Cláudio Mendes-Ribeiro, Paulo de Tarso Veras Farinatti, Tatiana M.C. Brunini, Cristiane Matsuura, Daniel J M Medeiros-Lima |
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| Rok vydání: | 2016 |
| Předmět: |
Adult
Male medicine.medical_specialty Erythrocytes Physiology 030204 cardiovascular system & hematology medicine.disease_cause Nitric Oxide Nitric oxide Lipid peroxidation Superoxide dismutase 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Physiology (medical) Internal medicine medicine Membrane fluidity Humans Exercise chemistry.chemical_classification Reactive oxygen species biology Superoxide Dismutase Glutathione peroxidase 030229 sport sciences Hematology Healthy Volunteers Nitric oxide synthase Oxidative Stress Endocrinology chemistry biology.protein Cardiology and Cardiovascular Medicine Reactive Oxygen Species Oxidation-Reduction Oxidative stress |
| Zdroj: | Clinical hemorheology and microcirculation. 65(3) |
| ISSN: | 1875-8622 |
| Popis: | Growing evidence has shown that acute exercise impairs erythrocyte membrane structure and function as a consequence of increased physical and chemical stress. Erythrocyte-synthesized nitric oxide (NO) is known to modulate membrane fluidity, and its bioavailability depends on the balance between its production and scavenging by reactive oxygen species. Here, we investigated whether a maximal exercise test could affect erythrocyte NO bioavailability and oxidative stress. Twelve men (26±4 years old, V̇O2peak 44.1±4.3 mL·kg-1·min-1) performed a treadmill maximal cardiopulmonary exercise test. Blood was collected at rest and immediately after exercise for erythrocytes isolation. Maximal exercise caused an increase in erythrocytes count, haemoglobin and haematocrit levels. There was no change in L-arginine influx into erythrocytes after exercise. Yet, nitric oxide synthase activity, and thus, NO production, was increased after maximal test, as well cyclic GMP levels. In relation to biomarkers of oxidative stress, maximal test resulted in increased levels of lipid peroxidation, and diminished superoxide dismutase activity. Neither glutathione peroxidase nor catalase activity was affected by maximal test. Our findings demonstrate that the increased erythrocyte membrane rigidity caused by an acute bout of exercise may be caused, in part, by an increased lipid oxidative damage caused by ROS produced exogenously. |
| Databáze: | OpenAIRE |
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