Mantle cell lymphoma cell lines show no evident immunoglobulin heavy chain stereotypy but frequent light chain stereotypy
| Autor: | Anna Bertolaso, Chiara Pighi, Alberto Zamò, Stefano Barbi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Cancer Research
Molecular Sequence Data Gene Rearrangement B-Lymphocyte Heavy Chain mantle cell lymphoma Lymphoma Mantle-Cell Complementarity determining region Biology stereotypy Immunoglobulin light chain Cell Line Tumor B-cell receptor medicine Cluster Analysis Gene Rearrangement B-Lymphocyte Light Chain Humans Amino Acid Sequence Genetics Hematology Gene rearrangement medicine.disease Complementarity Determining Regions Molecular biology Lymphoma immunogenetics Stereotypy (non-human) Oncology Mutation Immunoglobulin heavy chain Immunoglobulin Light Chains Mantle cell lymphoma Immunoglobulin Heavy Chains IGHV@ |
| Popis: | Mantle cell lymphoma shows a peculiar immunogenetic profile, but the functional consequences of this fact are unknown. We have determined the precise sequences of rearranged heavy and light chain genes in several mantle cell lymphoma cell lines and investigated the presence of heavy and light chain stereotypy. These cell lines use IGHV and IGLV genes that are known to be preferentially rearranged in mantle cell lymphoma, but we found no evidence of heavy chain stereotypy. In contrast, one cell line (Mino) showed a nearly identical light chain complementarity-determining region 3 when compared to the only published light chain cluster. Two cell line couples (Jeko-1/UPN-2 and JVM-2/JVM-13) showed a highly similar light chain that satisfied the criteria for stereotypy. Our data show that mantle cell lymphoma cell lines resemble the IGHV and IGLV usage of mantle cell lymphoma, and foster the hypothesis that light chain stereotypy might be under-recognized. |
| Databáze: | OpenAIRE |
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