Telemetric uterine contraction model in preterm cynomolgus monkeys
| Autor: | Julie K. Meyer, Torsten M. Reinheimer, George J. Haluska, David S. Moddrelle, John C. Resendez, Walter H. Bee, Jan Bernal, Gary J. Chellman |
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| Rok vydání: | 2003 |
| Předmět: |
Preterm labor
Uterus Electromyography Toxicology Oxytocin Uterine contraction Uterine Contraction Uterine Monitoring Catheters Indwelling Pregnancy Telemetry medicine Pressure Animals Amnion medicine.diagnostic_test business.industry Electrodes Implanted Macaca fascicularis medicine.anatomical_structure Anesthesia Models Animal Gestation Female medicine.symptom business Blood sampling |
| Zdroj: | Reproductive toxicology (Elmsford, N.Y.). 18(2) |
| ISSN: | 0890-6238 |
| Popis: | A telemetric-based model is presented for evaluation of uterine contractions and preterm labor (PTL) in pregnant cynomolgus monkeys. The model allows continuous monitoring of electromyography (EMG) and intrauterine pressure (IUP) as indicators of uterine activity. A pressure sensor was implanted into the amnion of pregnant monkeys on gestational day (GD) 120 +/- 3 and biopotential sensors were attached to the uterus. A telemetry transmitter was placed in a subcuticular pocket located in the flank. Venous catheters were tethered to the next room for dosing and blood sampling without disturbing the conscious animals. EMG and/or IUP were monitored continuously post-operatively. IUP is a reliable parameter for monitoring intrauterine activity, as demonstrated by a close relationship between bursts of activity in the EMG and increases in IUP. Animals close to term showed a basal level of uterine activity during the daytime, with irregular contractions of10 mmHg. In the night, spontaneous contractions (10-40 mmHg; maximum between 18:00 and 01:00 h) appeared every 3-6 min. Artificial contractions of 15-40 mmHg that mimicked preterm labor were induced at any time of the day by infusion of 5-60 mU oxytocin (OT) per kilogram per hour. These contractions showed a dose-dependent response to OT, and were stable for up to 14 h of constant infusion of OT. Following withdrawal of oxytocin, contractions returned to baseline within 1h. No desensitization of oxytocin-induced contractions was observed when oxytocin was administered daily for up to several weeks. This telemetric model characterizes uterine contractions in non-human primates and provides an excellent method to evaluate pharmacological characteristics of drug candidates intended to treat PTL. |
| Databáze: | OpenAIRE |
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