Emulsification and lipolysis of triacylglycerols are altered by viscous soluble dietary fibres in acidic gastric medium in vitro
| Autor: | Huguette Lafont, Chantal Castelain, Fabienne Guillon, Denis Lairon, Patrick Borel, Martine Armand, Bérengère Pasquier |
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| Přispěvatelé: | Centre de résonance magnétique biologique et médicale (CRMBM), Aix Marseille Université (AMU)-Assistance Publique - Hôpitaux de Marseille (APHM)-Centre National de la Recherche Scientifique (CNRS), Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA), Institut National de la Recherche Agronomique (INRA), Nutrition, obésité et risque thrombotique (NORT), Institut National de la Recherche Agronomique (INRA)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Unité de Recherche sur le Transport des Lipides, Institut National de la Santé et de la Recherche Médicale (INSERM), Centre recherche en CardioVasculaire et Nutrition = Center for CardioVascular and Nutrition research (C2VN), Centre recherche en CardioVasculaire et Nutrition (C2VN), Assistance Publique - Hôpitaux de Marseille (APHM)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), ProdInra, Migration, Laboratoire d'étude des interactions des molécules alimentaires, Laboratoire de technologie appliquée à la nutrition |
| Jazyk: | angličtina |
| Rok vydání: | 1996 |
| Předmět: |
Dietary Fiber
food.ingredient 030309 nutrition & dietetics TRIACYGLYCEROL Lipolysis Guar Biochemistry Galactans Gastric Acid Mannans 03 medical and health sciences chemistry.chemical_compound 0404 agricultural biotechnology food Phosphatidylcholine Plant Gums [SDV.BBM] Life Sciences [q-bio]/Biochemistry Molecular Biology Humans Gastric lipase [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Triolein Particle Size Molecular Biology ComputingMilieux_MISCELLANEOUS 0303 health sciences Guar gum Chromatography Gastric Juice Viscosity 04 agricultural and veterinary sciences Cell Biology Lipase Hydrogen-Ion Concentration Lipid Metabolism 040401 food science chemistry Solubility Emulsion Gum arabic Pectins Emulsions [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition Research Article |
| Zdroj: | Biochemical Journal Biochemical Journal, 1996, 314 (1), pp.269-275. ⟨10.1042/bj3140269⟩ Biochemical Journal, Portland Press, 1996, 314 (1), pp.269-275. ⟨10.1042/bj3140269⟩ Biochemical Journal, Portland Press, 1996, 314, pp.269-275 ResearcherID HAL |
| ISSN: | 0264-6021 1470-8728 |
| DOI: | 10.1042/bj3140269⟩ |
| Popis: | This in vitro study was designed to test the hypothesis that soluble dietary fibres can alter the process of intragastric lipid emulsification and possibly subsequent triacylglycerol lipolysis. Three guar gums, two pectins and gum arabic were dissolved in acidic gastric medium in the concentration range 0.3–2.0% (w/v). Viscosities of fibre solutions were measured and apparent viscosities varied over a wide range (0.7–77 mPa·s). Emulsification of a lipid mixture (triolein/phosphatidylcholine/cholesterol) was performed under mild conditions in the presence of increasing concentrations of soluble fibres. The amount of emulsified lipid was not affected whereas the size of the emulsified droplets was increased by raising the concentration of viscous fibres only. The droplet size (r=0.75, P=0.006) and overall droplet surface area (r=-0.69, P=0.009) were strongly correlated with the medium viscosity in the range 0–20 mPa·s. The addition of solutions of viscous fibres to a preformed standard emulsion did not change the initial velocity of human gastric lipase reaction. Conversely, when emulsions prepared in the presence of fibres (i.e. with different droplet sizes) were incubated with excess gastric enzyme for 2 h, the high-viscosity guar gum significantly reduced the extent of triacylglycerol lipolysis, as compared with control and low- or medium-viscosity fibres. In conclusion, the data obtained show that reducing emulsification of dietary lipids in the mildly acid medium found in the stomach is a mechanism by which soluble viscous fibres can alter lipid assimilation. |
| Databáze: | OpenAIRE |
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