Identification and Characterization of an Inducible NAD(P)H Dehydrogenase from Red Beetroot Mitochondria
| Autor: | David A. Day, R. I. Menz |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Dihydrolipoamide
Dihydrolipoamide dehydrogenase biology Physiology NADH dehydrogenase Dehydrogenase Plant Science NAD(P)H Dehydrogenase (Quinone) Molecular biology NADH dehydrogenase activity NAD(P)H dehydrogenase Glycerol-3-phosphate dehydrogenase Biochemistry Genetics medicine biology.protein Research Article medicine.drug |
| Zdroj: | Plant Physiology. 112:607-613 |
| ISSN: | 1532-2548 0032-0889 |
| Popis: | Exogenous NADH oxidation of mitochondria isolated from red beetroots (Beta vulgaris L.) increased dramatically upon slicing and aging the tissue. Anion-exchange chromatography of soluble fractions derived by sonication from fresh and aged beetroot mitochondria yielded three NADH dehydrogenase activity peaks. The third peak from aged beetroot mitochondria was separated into two activities by blue-affinity chromatography. One of these (the unbound peak) readily oxidized dihydrolipoamide, whereas the other (the bound peak) did not. The latter was an NAD(P)H dehydrogenase with high quinone and ferricyanide reductase activity and was absent from fresh beet mitochondria. Further affinity chromatography of the NAD(P)H dehydrogenase indicated enrichment of a 58-kD polypeptide on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. We propose that this 58-kD protein is the inducible, external NADH dehydrogenase. |
| Databáze: | OpenAIRE |
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