Intravenous Grafts of Human Amniotic Fluid-Derived Stem Cells Reduce Behavioral Deficits in Experimental Ischemic Stroke
| Autor: | Ivone Farias Cunha, Jardel Nicacio Mendonça, Alberto Tannus, Luciana Cavalheiro Marti, Jackeline Moraes Malheiros, Silvia Regina Caminada de Toledo, Francisco Romero Cabral, Suzana M. F. Malheiros, Fernando Fernandes Paiva, Lorena Favaro Pavon, Nelma Bastos Bezerra Rego, D. M. Oliveira, Marcos Devanir Silva da Costa, Sérgio Mascarenhas de Oliveira, Sergio Cavalheiro, Antonio Fernandes Moron, Jean Gabriel de Souza, Tatiana Tais Sibov, Manoel Antonio de Paiva Neto, Edgar Ferreira da Cruz, Patricia Alessandra Dastoli |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Adult
0301 basic medicine Pathology medicine.medical_specialty Amniotic fluid Cell human amniotic fluid-derived stem cells Biomedical Engineering Brain Ischemia Cell labeling ESTIMULAÇÃO CEREBRAL 03 medical and health sciences 0302 clinical medicine Pregnancy Animals Humans Medicine Rats Wistar Stroke cell culture Transplantation Behavior Animal business.industry intravenous grafts Stem Cells Original Articles Cell Biology Amniotic Fluid medicine.disease stroke Rats Disease Models Animal 030104 developmental biology medicine.anatomical_structure Cell culture Ischemic stroke Heterografts Female Stem cell business cell labeling 030217 neurology & neurosurgery Stem Cell Transplantation |
| Zdroj: | Cell Transplantation, Vol 28 (2019) Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Cell Transplantation |
| ISSN: | 1555-3892 0963-6897 |
| DOI: | 10.1177/0963689719854342 |
| Popis: | Amniotic fluid has been investigated as new cell source for stem cells in the development of future cell-based transplantation. This study reports isolation of viable human amniotic fluid-derived stem cells, labeled with multimodal iron oxide nanoparticles, and its effect on focal cerebral ischemia–reperfusion injury in Wistar rats. Middle cerebral artery occlusion of 60 min followed by reperfusion for 1 h, 6 h, and 24 h was employed in the present study to produce ischemia and reperfusion-induced cerebral injury in rats. Tests were employed to assess the functional outcome of the sensorimotor center activity in the brain, through a set of modified neurological severity scores used to assess motor and exploratory capacity 24 h, 14, and 28 days after receiving cellular therapy via tail vein. In our animal model of stroke, transplanted cells migrated to the ischemic focus, infarct volume decreased, and motor deficits improved. Therefore, we concluded that these cells appear to have beneficial effects on the ischemic brain, possibly based on their ability to enhance endogenous repair mechanisms. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|