Novel probiotics isolated from a Japanese traditional fermented food, Funazushi, attenuates DSS-induced colitis by increasing the induction of high integrin αv/β8-expressing dendritic cells
| Autor: | Soichiro Miura, Yoshikazu Tsuzuki, Shigeaki Nagao, Kengo Tomita, Kazuhiko Shirakabe, Chikako Watanabe, Masaaki Higashiyama, Takajo Takeshi, Chie Kurihara, Shunsuke Komoto, Hirotaka Furuhashi, Yoshikiyo Okada, Ryota Hokari |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Integrin beta Chains Integrin Anti-Inflammatory Agents CD11c Inflammation T-Lymphocytes Regulatory Microbiology Mice 03 medical and health sciences 0302 clinical medicine Japan Transforming Growth Factor beta Animals Medicine Large intestine RNA Messenger Colitis biology CD11 Antigens Tumor Necrosis Factor-alpha business.industry Probiotics Dextran Sulfate Gastroenterology hemic and immune systems Dendritic Cells Integrin alphaV medicine.disease Adoptive Transfer Mice Inbred C57BL RAW 264.7 Cells 030104 developmental biology medicine.anatomical_structure Immunology biology.protein Female 030211 gastroenterology & hepatology Tumor necrosis factor alpha Bone marrow Fermented Foods Antibody medicine.symptom business |
| Zdroj: | Journal of Gastroenterology. 53:407-418 |
| ISSN: | 1435-5922 0944-1174 |
| DOI: | 10.1007/s00535-017-1362-x |
| Popis: | We isolated two novel probiotics strains (s193 and s292) from Funazushi, which is a traditional Japanese fermented food, and evaluated its effects on DSS-induced colitis to determine the possible underlying mechanisms. A single colony from homogenized Funazushi was isolated by its ability to suppress TNF-α in RAW 264.7. Effect of probiotics on colonic inflammation induced by DSS was evaluated. Effect of probiotics on Treg induction by CD11c+ dendritic cells (DCs) of MLNs were analyzed. Two novel probiotics strains classified into the genus Lactobacillus were isolated (s193 and s292), and those strains showed stronger anti-inflammatory effects on DSS-induced colitis than those of L. gasseri isolated from the gut. mRNA expression β8 integrin in CD11c+DCs of MLNs and the number of Tregs in the large intestine were significantly increased by s193 and s292 administration compared with L. gasseri administration. Bone marrow DCs treated with s193 and s292 highly increased β8 integrin, and those cells strongly induced differentiation of CD4+ T cells into Tregs. Differentiation of Tregs was remarkably inhibited by anti-β8 integrin antibody treatment. Strains s193 and s292 demonstrate strong anti-inflammatory effects on DSS-induced colitis through induction of β8 integrin expression on DCs. Our results suggested that Japanese traditional fermented foods are valuable sources for probiotics that are effective for IBD therapy and treatment. |
| Databáze: | OpenAIRE |
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