Crenothrix are major methane consumers in stratified lakes
| Autor: | Jon S. Graf, Bernhard Wehrli, Michael Wagner, Jana Milucka, Carsten J. Schubert, Marcel M. M. Kuypers, Holger Daims, Mads Albertsen, Andreas Brand, Daniela Tienken, Sten Littmann, Kirsten Oswald |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Gammaproteobacteria/classification Methane monooxygenase Segmented filamentous bacteria 030106 microbiology Microbiology Sink (geography) Methane 03 medical and health sciences chemistry.chemical_compound Nitrate Bacterial Proteins Gammaproteobacteria Ecology Evolution Behavior and Systematics geography geography.geographical_feature_category biology Lakes/chemistry Ecology Bacterial Proteins/genetics Methane/analysis biology.organism_classification 6. Clean water Lakes Oxygenases/genetics chemistry 13. Climate action Greenhouse gas biology.protein Oxygenases Original Article Oxidation-Reduction Bacteria |
| Zdroj: | The ISME Journal, 11 (9) The ISME Journal Oswald, K, Graf, J S, Littmann, S, Tienken, D, Brand, A, Wehrli, B, Albertsen, M, Daims, H, Wagner, M, Kuypers, M M, Schubert, C J & Milucka, J 2017, ' Crenothrix are major methane consumers in stratified lakes ', I S M E Journal, vol. 11, no. 9, pp. 2124-2140 . https://doi.org/10.1038/ismej.2017.77 |
| ISSN: | 1751-7362 |
| DOI: | 10.1038/ismej.2017.77 |
| Popis: | Methane-oxidizing bacteria represent a major biological sink for methane and are thus Earth’s natural protection against this potent greenhouse gas. Here we show that in two stratified freshwater lakes a substantial part of upward-diffusing methane was oxidized by filamentous gamma-proteobacteria related to Crenothrix polyspora. These filamentous bacteria have been known as contaminants of drinking water supplies since 1870, but their role in the environmental methane removal has remained unclear. While oxidizing methane, these organisms were assigned an ‘unusual’ methane monooxygenase (MMO), which was only distantly related to ‘classical’ MMO of gamma-proteobacterial methanotrophs. We now correct this assignment and show that Crenothrix encode a typical gamma-proteobacterial PmoA. Stable isotope labeling in combination swith single-cell imaging mass spectrometry revealed methane-dependent growth of the lacustrine Crenothrix with oxygen as well as under oxygen-deficient conditions. Crenothrix genomes encoded pathways for the respiration of oxygen as well as for the reduction of nitrate to N2O. The observed abundance and planktonic growth of Crenothrix suggest that these methanotrophs can act as a relevant biological sink for methane in stratified lakes and should be considered in the context of environmental removal of methane. ISSN:1751-7362 ISSN:1751-7370 |
| Databáze: | OpenAIRE |
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