Spatiotemporal characterization of phagocytic NADPH oxidase and oxidative destruction of intraphagosomal organisms in vivo using autofluorescence imaging and Raman microspectroscopy
| Autor: | Wei-Tien Chang, I-Lin Li, Yi-Cyun Yang, Ian Liau, Hsueh-Han Lu |
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| Rok vydání: | 2010 |
| Předmět: |
Time Factors
Cell Survival Confocal Phagocytosis Oxidative phosphorylation Spectrum Analysis Raman Biochemistry Catalysis Colloid and Surface Chemistry In vivo Phagosomes Oxidase test NADPH oxidase biology Chemistry Macrophages NADPH Oxidases General Chemistry Raman microspectroscopy Molecular Imaging Autofluorescence Microscopy Fluorescence biology.protein Reactive Oxygen Species Oxidation-Reduction |
| Zdroj: | Journal of the American Chemical Society. 132(6) |
| ISSN: | 1520-5126 |
| Popis: | The ability to generate antimicrobial reactive-oxygen species (ROS) by reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase is critical for the host to defend against invading microbes. We have demonstrated the application of confocal autofluorescence microscopy and Raman microspectroscopy to characterize dynamically the phagocytic process of living macrophages in a label-free manner. In particular, we visualized the translocation of NADPH oxidases in living macrophages that are undergoing phagocytosis of invading yeasts and monitored dynamically the change at the molecular level of single intraphagosomal yeasts caused by phagocytic ROS. |
| Databáze: | OpenAIRE |
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