Inhibition of hedgehog signaling promotes white adipose tissue browning
| Autor: | Zhi Feng Liu, Zhuo Zhang, Xi Rong Guo, Chen Bo Ji, Xian wei Cui, Xiaoxiao Zhang, Xia Chi, Hong Zhong |
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| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine Agonist medicine.drug_class Adipose Tissue White Primary Cell Culture 030209 endocrinology & metabolism Dioxoles White adipose tissue Zinc Finger Protein GLI1 Biochemistry Mice Norepinephrine 03 medical and health sciences 0302 clinical medicine Endocrinology Adipose Tissue Brown GLI1 Adipocytes Browning medicine Animals Humans Hedgehog Proteins Molecular Biology Hedgehog Adipogenesis biology Chemistry Cell Differentiation Thermogenesis Smoothened Receptor Hedgehog signaling pathway Cell biology Cold Temperature Repressor Proteins 030104 developmental biology Gene Expression Regulation biology.protein Energy Metabolism Smoothened Signal Transduction |
| Zdroj: | Molecular and Cellular Endocrinology. 518:110970 |
| ISSN: | 0303-7207 |
| Popis: | White adipose tissue (WAT) browning is a potential strategy to treat obesity, and is characterized by the formation of brown adipocytes induced by cold or β-3 adrenergic receptor (β-3AR) agonist treatment. The hedgehog (Hh) signaling at the primary cilium is closely related to obesity, and plays a key role in the differentiation and adipogenesis of adipocytes. However, little is known about its effects on WAT browning. In this study, browning models were used to evaluate the activity and effect of Hh signaling on WAT browning using Hh antagonists, agonist, and small-interfering RNAs (siRNAs) specific for glioma-associated oncogene homologue 1 (Gli1), smoothened (Smo), and suppressor of fused (Sufu). We observed that Hh signaling activity was inhibited during the browning process both in vivo and in vitro. Further, Hh signaling inhibition enhanced WAT browning, while its activation attenuated norepinephrine-induced browning. Thus, the inhibition of Hh signaling promotes WAT browning and therefore, Hh signaling may be a therapeutic target against obesity and associated comorbidities. |
| Databáze: | OpenAIRE |
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