Inhibition of pancreatic carboxypeptidase A: A possible mechanism of interaction between penicillic acid and ochratoxin A
| Autor: | N. D. Heidelbaugh, Roger W. Parker, Leon H. Russell, Leon F. Kubena, Timothy D. Phillips |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Male
Ochratoxin A Time Factors Carboxypeptidases A Metabolite Carboxypeptidases In Vitro Techniques Mice chemistry.chemical_compound Species Specificity In vivo Penicillic acid Animals Drug Interactions Mycotoxin Caproates Pancreas Penicillic Acid Chromatography High Pressure Liquid chemistry.chemical_classification biology food and beverages General Medicine Ochratoxins Pollution In vitro Enzyme Biochemistry chemistry Carboxypeptidase A biology.protein Chickens Food Science |
| Zdroj: | Journal of Environmental Science and Health, Part B. 17:77-91 |
| ISSN: | 1532-4109 0360-1234 |
| DOI: | 10.1080/03601238209372304 |
| Popis: | Penicillic acid and ochratoxin A are environmentally important toxic fungal metabolites (mycotoxins) that are synergistic in combination. The effects of penicillic acid on the pancreatic enzyme, :arboxypeptidase A were investigated in vitro and in vivo. A broad range of inhibition in vitro of the enzyme by PA was demonstrated with a half‐maximal inhibitory concentration equal to 1.1 x 10‐4M PA. Inhibition of carboxypeptidase A was time and temperature dependent, and resulted in decreased conversion of parent ochratoxin A to the non‐toxic metabolite, ochratoxin alpha. Studies in vivo demonstrated a penicillic acid‐dependent inhibition of pancreatic carboxypeptidase A activity in the mouse and the chicken following multiple oral exposure. It is postulated that the mode of toxic interaction of the two mycotoxins may be due, in part, to impaired detoxification of ochratoxin A through peni‐cillic acid depletion of carboxypeptidase A activity. |
| Databáze: | OpenAIRE |
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