RNA Docking and Local Translation Regulate Site-Specific Axon Remodeling In Vivo
| Autor: | Holt, CE, Wong, H-W, Lin, Q, Stroehl, F, Roque, CG, Cioni, JM, Cagnetta, R, Turner-Bridger, B, Laine, R, Harris, WA, Kaminski, CF |
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| Přispěvatelé: | Holt, Christine [0000-0003-2829-121X], Wong, Hovy [0000-0003-3317-478X], Lin, Qiaojin [0000-0002-2669-6478], Stroehl, Florian [0000-0002-2603-0780], Turner-Bridger, Benita [0000-0003-3718-3632], Harris, William [0000-0002-9995-8096], Kaminski, Clemens [0000-0002-5194-0962], Apollo - University of Cambridge Repository |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Blastomeres
Embryo Nonmammalian neural wiring Biotin In Vitro Techniques Retina Morpholinos Xenopus laevis Organ Culture Techniques retinotectal projection Animals local protein synthesis β-actin Cycloheximide RNA trafficking Protein Synthesis Inhibitors RNA labeling axon guidance fungi Gene Expression Regulation Developmental RNA localization Carbocyanines Oligonucleotides Antisense Actins Axons Mitochondria axon branching Luminescent Proteins nervous system FRAP RNA Deoxyuracil Nucleotides Anisomycin |
| DOI: | 10.17863/cam.11811 |
| Popis: | Nascent proteins can be positioned rapidly at precise subcellular locations by local protein synthesis (LPS) to facilitate localized growth responses. Axon arbor architecture, a major determinant of synaptic connectivity, is shaped by localized growth responses, but it is unknown whether LPS influences these responses in vivo. Using high-resolution live imaging, we examined the spatiotemporal dynamics of RNA and LPS in retinal axons during arborization in vivo. Endogenous RNA tracking reveals that RNA granules dock at sites of branch emergence and invade stabilized branches. Live translation reporter analysis reveals that de novo ß-actin hotspots colocalize with docked RNA granules at the bases and tips of new branches. Inhibition of axonal ß-actin mRNA translation disrupts arbor dynamics primarily by reducing new branch emergence and leads to impoverished terminal arbors. The results demonstrate a requirement for LPS in building arbor complexity and suggest a key role for pre-synaptic LPS in assembling neural circuits. |
| Databáze: | OpenAIRE |
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