Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxyhexanoate) by metabolically engineeredEscherichia coli strains
| Autor: | Sang Yup Lee, Si Jae Park, Woo Suk Ahn, Phillip Richard Green |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Aeromonas caviae
Polymers Bioengineering medicine.disease_cause Applied Microbiology and Biotechnology Polyhydroxyalkanoates Metabolic engineering Escherichia coli medicine Enoyl-CoA Hydratase chemistry.chemical_classification biology Lauric Acids Fatty acid biology.organism_classification Enterobacteriaceae Aeromonas hydrophila Aeromonas Biochemistry chemistry Fermentation Genetic Engineering Acyltransferases Biotechnology |
| Zdroj: | Biotechnology and Bioengineering. 74:82-87 |
| ISSN: | 1097-0290 0006-3592 |
| DOI: | 10.1002/bit.1097 |
| Popis: | The recombinant Escherichia coli strain, equipped with the newly cloned Aeromonas PHA biosynthesis genes, could produce a terpolymer of 3-hydroxybutyrate (3HB), 3-hydroxyvalerate (3HV), and 3-hydroxyhexanoate (3HHx) [P(3HB-co-3HV-co-3HHx)] from dodecanoic acid plus odd carbon number fatty acid. In addition, the orf1 gene of Aeromonas hydrophila was found to play a critical role in assimilating the 3HV monomer and in regulating the monomer fraction in the terpolymer. |
| Databáze: | OpenAIRE |
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