Characterization of the psbH precursor RNAs reveals a precise endoribonuclease cleavage site in the psbT/psbH intergenic region that is dependent on psbN gene expression

Autor: Damien Rondet, Livia Merendino, Thomas Pfannschmidt, Silva Lerbs-Mache, Mustafa Malik Ghulam, Fabien Chevalier
Přispěvatelé: Laboratoire de physiologie cellulaire végétale (LPCV), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Recherche Agronomique (INRA)-Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS), Grenoble Alliance for Integrated Structural Cell Biology- Higher Education Commission of Pakistan- Centre National de la Recherche Scientifique (CNRS), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Recherche Agronomique (INRA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Recherche Agronomique (INRA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)
Jazyk: angličtina
Rok vydání: 2015
Předmět:
Chloroplasts
Endoribonuclease activity
[SDV]Life Sciences [q-bio]
Arabidopsis
Plant Science
MESH: Base Sequence
MESH: RNA
Plant
Transcription (biology)
Sense (molecular biology)
Gene expression
RNA Precursors
MESH: Genes
Plant
MESH: Arabidopsis
MESH: RNA
Antisense
RNA Processing
Post-Transcriptional
Antisense RNA
Genetics
MESH: Photosystem II Protein Complex
General Medicine
Plants
Genetically Modified
RNA
Plant
DNA
Intergenic
MESH: RNA Processing
Post-Transcriptional
RNA Processing
MESH: Endoribonucleases
MESH: Gene Expression
MESH: Mutation
Endoribonuclease
Molecular Sequence Data
MESH: RNA Precursors
MESH: Arabidopsis Proteins
Biology
Genes
Plant
Chloroplast
MESH: Phosphoproteins
Endoribonucleases
RNA
Antisense
[SDV.BBM]Life Sciences [q-bio]/Biochemistry
Molecular Biology
MESH: DNA
Intergenic
Binding Sites
MESH: Chloroplasts
MESH: Molecular Sequence Data
Base Sequence
Arabidopsis Proteins
RNA
Photosystem II Protein Complex
Plant
Phosphoproteins
MESH: Binding Sites
MESH: Plants
Genetically Modified
Mutation
RNA Cleavage
Endonucleolytic cleavage
Agronomy and Crop Science
Zdroj: Plant Molecular Biology Reporter
Plant Molecular Biology Reporter, Springer Verlag, 2015, 88 (4-5), pp.357-67. ⟨10.1007/s11103-015-0325-y⟩
Plant Molecular Biology Reporter, Springer Verlag, 2015, 88 (4-5), pp.357-67
Plant Molecular Biology Reporter, 2015, 88 (4-5), pp.357-67. ⟨10.1007/s11103-015-0325-y⟩
ISSN: 0735-9640
DOI: 10.1007/s11103-015-0325-y⟩
Popis: International audience; The plastid psbB operon harbours 5 genes, psbB, psbT, psbH, petB and petD. A sixth gene, the psbN gene, is located on the opposite DNA strand in the psbT/psbH intergenic region. Its transcription produces antisense RNA to a large part of the psbB pentacistronic mRNA. We have investigated whether transcription of the psbN gene, i.e. production of antisense RNA, influences psbT/psbH intergenic processing. Results reveal the existence of four different psbH precursor RNAs. Three of them result from processing and one is produced by transcription initiation. One of the processed RNAs is probably created by site-specific RNA cleavage. This RNA is absent in plants where the psbN gene is not transcribed suggesting that cleavage at this site is dependent on the formation of sense/antisense double-stranded RNA. In order to characterize the nuclease that might be responsible for double-stranded RNA cleavage, we analysed csp41a and csp41b knock-out mutants and the corresponding double mutant. Both CSP41 proteins are known to interact physically and CSP41a had been shown to cleave within 3'-untranslated region stem-loop structures, which contain double-stranded RNA, in vitro. We demonstrate that the psbH RNA, that is absent in plants where the psbN gene is not transcribed, is also strongly diminished in all csp41 plants. Altogether, results reveal a site-specific endoribonuclease cleavage event that seems to depend on antisense RNA and might implicate endoribonuclease activity of CSP41a.
Databáze: OpenAIRE
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