Sex-related effects on diabetes-induced alterations in calcium release in the rat heart

Autor: Belma Turan, Guy Vassort, Nazmi Yaras, Nuhan Purali, Babur Sahinoglu, Erkan Tuncay
Rok vydání: 2007
Předmět:
Male
medicine.medical_specialty
Time Factors
Calcium Channels
L-Type
Physiology
chemistry.chemical_element
Calcium
medicine.disease_cause
Ventricular Function
Left
Diabetes Mellitus
Experimental
Tacrolimus Binding Proteins
Sex Factors
Risk Factors
Physiology (medical)
Internal medicine
Diabetes mellitus
Ventricular Pressure
medicine
Animals
Myocytes
Cardiac
Calcium Signaling
Sulfhydryl Compounds
Phosphorylation
Rats
Wistar
Protein Kinase C
Calcium signaling
Type 1 diabetes
Ryanodine receptor
business.industry
Myocardium
Ryanodine Receptor Calcium Release Channel
medicine.disease
Rats
Calcium sparks
Oxidative Stress
Protein Transport
Sarcoplasmic Reticulum
Endocrinology
chemistry
Cardiovascular Diseases
Circulatory system
Female
Cardiology and Cardiovascular Medicine
business
Oxidative stress
Zdroj: American Journal of Physiology-Heart and Circulatory Physiology. 293:H3584-H3592
ISSN: 1522-1539
0363-6135
DOI: 10.1152/ajpheart.00619.2007
Popis: The present study was designed to determine whether the properties of local Ca2+release and its related regulatory mechanisms might provide insight into the role of sex differences in heart functions of control and streptozotocin-induced diabetic adult rats. Left ventricular developed pressure, the rates of pressure development and decay (±dP/d t), basal intracellular Ca2+level ([Ca2+]i), and spatiotemporal parameters of [Ca2+]itransients were found to be similar in male and female control rats. However, spatiotemporal parameters of Ca2+sparks in cardiomyocytes isolated from control females were significantly larger and slower than those in control males. Diabetes reduced left ventricular developed pressure to a lower extent in females than in males, and the diabetes-induced depressions in both +dP/d t and −dP/d t were less in females than in males. Diabetes elicited a smaller reduction in the amplitude of [Ca2+]itransients in females than in males, a smaller reduction in sarcoplasmic reticulum-Ca2+load, and less increase in basal [Ca2+]i. Similarly, the elementary Ca2+events and their control proteins were clearly different in both sexes, and these differences were more marked in diabetes. Diabetes-induced depression of the Ca2+spark amplitude was significantly less in females than in matched males. Levels of cardiac ryanodine receptors (RyR2) and FK506-binding protein 12.6 in control females were significantly higher than those shown in control males. Diabetes induced less RyR2 phosphorylation and FK506-binding protein 12.6 unbinding in females. Moreover, total and free sulfhydryl groups were significantly less reduced, and PKC levels were less increased, in diabetic females than in diabetic males. The present data related to local Ca2+release and its related proteins describe some of the mechanisms that may underlie sex-related differences accounting for females to have less frequent development of cardiac diseases.
Databáze: OpenAIRE
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