Novel Phenolic Constituents of Pulmonaria officinalis L. LC-MS/MS Comparison of Spring and Autumn Metabolite Profiles
| Autor: | Agnieszka Ludwiczuk, Mariusz Kowalczyk, Justyna Krzyżanowska-Kowalczyk, Jaroslaw Moldoch, Łukasz Pecio |
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| Rok vydání: | 2018 |
| Předmět: |
Metabolite
Pharmaceutical Science Mass spectrometry 01 natural sciences multivariate analyses Analytical Chemistry lcsh:QD241-441 Pulmonaria officinalis Pulmonariae Herba chemistry.chemical_compound danshensu/caffeic acid/rosmarinic acid derivatives seasonal variability lcsh:Organic chemistry Drug Discovery Lc ms ms Caffeic acid Physical and Theoretical Chemistry Chromatography biology lungwort 010405 organic chemistry Chemistry Rosmarinic acid 010401 analytical chemistry Organic Chemistry Nuclear magnetic resonance spectroscopy Boraginaceae HR-QTOF/MS biology.organism_classification NMR CD 0104 chemical sciences Chemistry (miscellaneous) metabolite profiling Molecular Medicine |
| Zdroj: | Molecules, Vol 23, Iss 9, p 2277 (2018) Molecules Volume 23 Issue 9 |
| ISSN: | 1420-3049 |
| DOI: | 10.3390/molecules23092277 |
| Popis: | Lungwort (Pulmonaria officinalis L., Boraginaceae) is considered to possess therapeutic properties and it has been traditionally used as a remedy against various lung disorders in many countries. Nevertheless, very few data concerning its phytochemical composition are available. This research aims to provide a detailed description of specialized metabolites from the aerial parts of lungwort. Nine previously undescribed and 36 known phenolic compounds were detected in the 50% methanolic extract. Following multistep preparative procedures, structures of newly discovered compounds were determined using one- and two-dimensional techniques of NMR spectroscopy. Among the identified compounds were caffeic acid esters with aliphatic hydroxycarboxylic acids, conjugates of dicaffeic acid with rosmarinic acid, and previously unknown isomers of isosalvianolic acid A and yunnaneic acid E, as well as other lignans. Concentrations of all identified phenolic derivatives in the investigated herbal material were estimated using a method based on liquid chromatography with high-resolution mass spectrometry detection. Seasonal changes in the concentration of metabolites were also investigated using targeted and untargeted metabolomics techniques. |
| Databáze: | OpenAIRE |
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