Towards the profiling of the Arabidopsis thaliana plasma membrane transportome by targeted proteomics
| Autor: | Véronique Santoni, Sonia Hem, Madeleine Sugano, Thierry Becue, Jean-Marc Monneuse, Michel Rossignol |
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| Přispěvatelé: | Biochimie et Physiologie Moléculaire des Plantes (BPMP), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Unité de Recherche Protéomique (PROTEOMIQUE), Institut National de la Recherche Agronomique (INRA), Université de Montpellier (UM)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Protéomique Fonctionnelle (LPF) |
| Rok vydání: | 2011 |
| Předmět: |
Proteomics
0106 biological sciences Gene isoform ATPase Molecular Sequence Data Arabidopsis Transporter 01 natural sciences Biochemistry Mass Spectrometry Transcriptome 03 medical and health sciences Gene Expression Regulation Plant Protein Isoforms [SDV.BV]Life Sciences [q-bio]/Vegetal Biology Amino Acid Sequence Molecular Biology 030304 developmental biology 0303 health sciences biology Arabidopsis Proteins Gene Expression Profiling Cell Membrane Membrane Transport Proteins biology.organism_classification Proton pump Plant Leaves Membrane biology.protein MRM Peptides Chromatography Liquid Plasma membrane 010606 plant biology & botany |
| Zdroj: | Proteomics Proteomics, Wiley-VCH Verlag, 2011, 11 (9), pp.1789-97. ⟨10.1002/pmic.201000660⟩ |
| ISSN: | 1615-9853 1615-9861 |
| DOI: | 10.1002/pmic.201000660 |
| Popis: | International audience; Plant membranes bear a variety of transporters belonging to multigene families that are affected by environmental and nutritional conditions. In addition, they often display high-sequence identity, making difficult in-depth investigation by current shot-gun strategies. In this study, we set up a targeted proteomics approach aimed at identifying and quantifying within single experiments the five major proton pumps of the autoinhibited H(+) ATPases (AHA) family, the 13 plasma membrane intrinsic proteins (PIP) water channels (PIPs), and ten members of ammonium transporters (AMTs) and nitrate transporter (NRT) families. Proteotypic peptides were selected and isotopically labeled heavy versions were used for technical optimization and for quantification of the corresponding light version in biological samples. This approach allowed to quantify simultaneously nine PIPs in leaf membranes and 13 PIPs together with three autoinhibited H(+) ATPases, two ammonium transporters, and two NRTs in root membranes. Similarly, it was used to investigate the effect of a salt stress on the expression of these latter 20 transporters in roots. These novel isoform-specific data were compared with published transcriptome information and revealed a close correlation between PIP isoforms and transcripts levels. The obtained resource is reusable and can be expanded to other transporter families for large-scale profiling of membrane transporters. |
| Databáze: | OpenAIRE |
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