Rescue of Caenorhabditis elegans pharyngeal development by a vertebrate heart specification gene
| Autor: | Peter G. Okkema, Christina Haun, Didier Y.R. Stainier, Jonathan Alexander |
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| Rok vydání: | 1998 |
| Předmět: |
animal structures
Molecular Sequence Data Biology Xenopus Proteins Homeobox protein Nkx-2.5 Pharyngeal muscles medicine Animals Amino Acid Sequence Caenorhabditis elegans Caenorhabditis elegans Proteins Zebrafish Genetics Regulation of gene expression Homeodomain Proteins Multidisciplinary Genetic Complementation Test Cardiac muscle Genes Homeobox Gene Expression Regulation Developmental Heart Biological Sciences biology.organism_classification medicine.anatomical_structure embryonic structures Homeobox Protein Nkx-2.5 Homeobox Pharynx Ectopic expression Transcription Factors |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America. 95(9) |
| ISSN: | 0027-8424 |
| Popis: | Development of pharyngeal muscle in nematodes and cardiac muscle in vertebrates and insects involves the related homeobox genes ceh-22 , nkx2.5 , and tinman , respectively. To determine whether the nematode and vertebrate genes perform similar functions, we examined activity of the zebrafish nkx2.5 gene in transgenic Caenorhabditis elegans . Here, we report that ectopic expression of nkx2.5 in C. elegans body wall muscle can directly activate expression of both the endogenous myo-2 gene, a ceh-22 target normally expressed only in pharyngeal muscle, and a synthetic reporter construct controlled by a multimerized CEH-22 binding site. nkx2.5 also efficiently rescues a ceh-22 mutant when expressed in pharyngeal muscle. Together, these results indicate that nkx2.5 and ceh-22 provide a single conserved molecular function. Further, they suggest that an evolutionarily conserved mechanism underlies heart development in vertebrates and insects and pharyngeal development in nematodes. |
| Databáze: | OpenAIRE |
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